Mostra el registre d'ítem simple

dc.contributor.authorHoyos Nogués, Mireia
dc.contributor.authorBuxadera Palomero, Judit
dc.contributor.authorGinebra Molins, Maria Pau
dc.contributor.authorManero Planella, José María
dc.contributor.authorGil, F. J.
dc.contributor.authorMas Moruno, Carlos
dc.contributor.otherUniversitat Politècnica de Catalunya. Departament de Ciència dels Materials i Enginyeria Metal·lúrgica
dc.date.accessioned2018-07-13T07:46:38Z
dc.date.available2018-07-13T07:46:38Z
dc.date.issued2018-09-01
dc.identifier.citationHoyos, M., Buxadera-Palomero, J., Ginebra, M.P., Manero, J., Gil, F., Mas-Moruno, C. All-in-one trifunctional strategy: A cell adhesive, bacteriostatic and bactericidal coating for titanium implants. "Colloids and Surfaces B: Biointerfaces", 1 Setembre 2018, vol. 169, p. 30-40.
dc.identifier.issn1873-4367
dc.identifier.urihttp://hdl.handle.net/2117/119295
dc.description.abstractStrategies to inhibit initial bacterial adhesion are extremely important to prevent infection on biomaterial surfaces. However, the simultaneous attraction of desired eukaryotic cells remains a challenge for successful biomaterial-host tissue integration. Here we describe a method for the development of a trifunctional coating that repels contaminating bacteria, kills those that adhere, and promotes osteoblast adhesion. To this end, titanium surfaces were functionalized by electrodeposition of an antifouling polyethylene glycol (PEG) layer and subsequent binding of a peptidic platform with cell-adhesive and bactericidal properties. The physicochemical characterization of the samples via SEM, contact angle, FTIR and XPS analysis verified the successful binding of the PEG layer and the biomolecules, without altering the morphology and topography of the samples. PEG coatings inhibited protein adsorption and osteoblast-like (SaOS-2) attachment; however, the presence of cell adhesive domains rescued osteoblast adhesion, yielding higher values of cell attachment and spreading compared to controls (p < 0.05). Finally, the antibacterial potential of the coating was measured by live/dead assays and SEM using S. sanguinis as a model of early colonizer in oral biofilms. The presence of PEG layers significantly reduced bacterial attachment on the surfaces (p < 0.05). This antibacterial potential was further increased by the bactericidal peptide, yielding values of bacterial adhesion below 0.2% (p < 0.05). The balance between the risk of infection and the optimal osteointegration of a biomaterial is often described as “the race for the surface”, in which contaminating bacteria and host tissue cells compete to colonize the implant. In the present work, we have developed a multifunctional coating for a titanium surface that promotes the attachment and spreading of osteoblasts, while very efficiently inhibits bacterial colonization, thus holding promise for application in bone replacing applications.
dc.format.extent11 p.
dc.language.isoeng
dc.subjectÀrees temàtiques de la UPC::Enginyeria dels materials
dc.subject.lcshTitanium
dc.subject.lcshPeptides
dc.subject.lcshAntibacterial agents
dc.subject.lcshPolyethylene glycol
dc.subject.lcshOsseointegration
dc.titleAll-in-one trifunctional strategy: A cell adhesive, bacteriostatic and bactericidal coating for titanium implants
dc.typeArticle
dc.subject.lemacTitani
dc.subject.lemacPèptids
dc.contributor.groupUniversitat Politècnica de Catalunya. BBT - Biomaterials, Biomecànica i Enginyeria de Teixits
dc.identifier.doi10.1016/j.colsurfb.2018.04.050
dc.description.peerreviewedPeer Reviewed
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0927776518302613
dc.rights.accessOpen Access
local.identifier.drac23170096
dc.description.versionPostprint (author's final draft)
local.citation.authorHoyos, M.; Buxadera-Palomero, J.; Ginebra, M.P.; Manero, J.; Gil, F.; Mas-Moruno, C.
local.citation.publicationNameColloids and Surfaces B: Biointerfaces
local.citation.volume169
local.citation.startingPage30
local.citation.endingPage40


Fitxers d'aquest items

Thumbnail

Aquest ítem apareix a les col·leccions següents

Mostra el registre d'ítem simple

Tots els drets reservats. Aquesta obra està protegida pels drets de propietat intel·lectual i industrial corresponents. Sense perjudici de les exempcions legals existents, queda prohibida la seva reproducció, distribució, comunicació pública o transformació sense l'autorització del titular dels drets