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dc.contributor.authorSrinivasan, Sundaramoorthy
dc.contributor.authorFernández Sampedro, Miguel Ángel
dc.contributor.authorMorillo Cazorla, Margarita
dc.contributor.authorRamon Portés, Eva
dc.contributor.authorJiménez Rosés, Mireia
dc.contributor.authorCordomi, Arnau
dc.contributor.authorGarriga Solé, Pere
dc.contributor.otherUniversitat Politècnica de Catalunya. Departament d'Enginyeria Química
dc.date.accessioned2018-06-11T11:38:25Z
dc.date.available2019-03-27T01:30:48Z
dc.date.issued2018-03-27
dc.identifier.citationSrinivasan, S., Fernández-Sampedro, M. A., Morillo, M., Ramon, E., Jiménez-Rosés, M., Cordomi, A., Garriga, P. Human blue cone opsin regeneration involves secondary retinal binding with analog specificity. "Biophysical journal", 27 Març 2018, vol. 114, núm. 6, p. 1285-1294.
dc.identifier.issn0006-3495
dc.identifier.urihttp://hdl.handle.net/2117/118008
dc.description.abstractHuman color vision is mediated by the red, green, and blue cone visual pigments. Cone opsins are G-protein-coupled receptors consisting of an opsin apoprotein covalently linked to the 11-cis-retinal chromophore. All visual pigments share a common evolutionary origin, and red and green cone opsins exhibit a higher homology, whereas blue cone opsin shows more resemblance to the dim light receptor rhodopsin. Here we show that chromophore regeneration in photoactivated blue cone opsin exhibits intermediate transient conformations and a secondary retinoid binding event with slower binding kinetics. We also detected a fine-tuning of the conformational change in the photoactivated blue cone opsin binding site that alters the retinal isomer binding specificity. Furthermore, the molecular models of active and inactive blue cone opsins show specific molecular interactions in the retinal binding site that are not present in other opsins. These findings highlight the differential conformational versatility of human cone opsin pigments in the chromophore regeneration process, particularly compared to rhodopsin, and point to relevant functional, unexpected roles other than spectral tuning for the cone visual pigments
dc.format.extent10 p.
dc.language.isoeng
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Spain
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.subjectÀrees temàtiques de la UPC::Ciències de la visió::Biologia ocular
dc.subject.lcshColor vision
dc.subject.lcshG proteins -- Receptors
dc.subject.lcshLigands (Biochemistry)
dc.subject.otherColor vision
dc.subject.otherG protein-coupled receptor
dc.subject.otherRetinoid
dc.subject.otherLigand binding
dc.subject.otherAllostery
dc.subject.otherSpectroscopy
dc.titleHuman blue cone opsin regeneration involves secondary retinal binding with analog specificity
dc.typeArticle
dc.subject.lemacProteïnes G -- Receptors
dc.subject.lemacSentit del color
dc.subject.lemacLligands (Bioquímica)
dc.contributor.groupUniversitat Politècnica de Catalunya. GBMI - Grup de Biotecnologia Molecular i Industrial
dc.identifier.doi10.1016/j.bpj.2018.01.032
dc.description.peerreviewedPeer Reviewed
dc.relation.publisherversionhttp://www.cell.com/biophysj/fulltext/S0006-3495(18)30191-7
dc.rights.accessOpen Access
local.identifier.drac22421832
dc.description.versionPostprint (author's final draft)
local.citation.authorSrinivasan, S.; Fernández-Sampedro, M. A.; Morillo, M.; Ramon, E.; Jiménez-Rosés, M.; Cordomi, A.; Garriga, P.
local.citation.publicationNameBiophysical journal
local.citation.volume114
local.citation.number6
local.citation.startingPage1285
local.citation.endingPage1294


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Attribution-NonCommercial-NoDerivs 3.0 Spain
Except where otherwise noted, content on this work is licensed under a Creative Commons license : Attribution-NonCommercial-NoDerivs 3.0 Spain