The diversity of sexual behaviors is driven by multiple determinants, including physiological, cultural, educational, and sociological factors. However, the definition of sexual behavior profiles has been barely addressed from a comprehensive point of view. We aimed to develop a multidisciplinary questionnaire for defining individual sexual behavior profiles. The questionnaire was developed by a panel of experts with research experience in the fields of urology, gynecology, psychology, anthropology, and sexology. The list of items was defined in a focus group session and was based on four categories—family-oriented, loving, recreational, and functional—resulting from the combination of two axes: traditional versus non-traditional and sexual benefit versus extra-sexual benefit. Real-time Delphi dynamics was used to assign a weight to each question and a bias to the corresponding responses. The final questionnaire included 50 items considered relevant for describing sexual profiles; the final questionnaire was named SEX360. Of the 50 items included in SEX360, 14 were considered essential for computing the final score; 9 of them were associated with 2 categories, 4 of them with 4 categories, and 1 of them with 3. Nine items referred to the category “family-oriented”, 10 to “loving”, 8 to “recreational”, and 9 to “functional”. The weights assigned to each question ranged from 3.00 to 4.33, and the centers of gravity ranged from 1 to 4. The questionnaire proposed shows the existence of a vast diversity of sexual behavior profiles and may serve as a tool for sexual behavior research.