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dc.contributor.authorLewin, Susanne
dc.contributor.authorBarba Martí, Antonio
dc.contributor.authorPersson, Cecilia
dc.contributor.authorFranch Serracanta, Jordi
dc.contributor.authorGinebra Molins, Maria Pau
dc.contributor.authorOhman, Caroline
dc.contributor.otherUniversitat Politècnica de Catalunya. Departament d'Enginyeria Telemàtica
dc.contributor.otherUniversitat Politècnica de Catalunya. Departament de Ciència dels Materials i Enginyeria Metal·lúrgica
dc.date.accessioned2018-01-25T18:08:59Z
dc.date.available2018-10-05T00:30:42Z
dc.date.issued2017-12-01
dc.identifier.citationLewin, S., Barba, A., Persson, C., Franch Serracanta, Jordi, Ginebra, M.P., Ohman, C. Evaluation of bone formation in calcium phosphate scaffolds with mu CT-method validation using SEM. "Biomedical materials", 1 Desembre 2017, vol. 12, núm. 6.
dc.identifier.issn1748-6041
dc.identifier.urihttp://hdl.handle.net/2117/113219
dc.description.abstractThere is a plethora of calcium phosphate (CaP) scaffolds used as synthetic substitutes to bone grafts. The scaffold performance is often evaluated from the quantity of bone formed within or in direct contact with the scaffold. Micro-computed tomography (µCT) allows three-dimensional evaluation of bone formation inside scaffolds. However, the almost identical x-ray attenuation of CaP and bone obtrude the separation of these phases in µCT images. Commonly, segmentation of bone in µCT images is based on gray scale intensity, with manually determined global thresholds. However, image analysis methods, and methods for manual thresholding in particular, lack standardization and may consequently suffer from subjectivity. The aim of the present study was to provide a methodological framework for addressing these issues. Bone formation in two types of CaP scaffold architectures (foamed and robocast), obtained from a larger animal study (a 12 week canine animal model) was evaluated by µCT. In addition, cross-sectional scanning electron microscopy (SEM) images were acquired as references to determine thresholds and to validate the result. µCT datasets were registered to the corresponding SEM reference. Global thresholds were then determined by quantitatively correlating the different area fractions in the µCT image, towards the area fractions in the corresponding SEM image. For comparison, area fractions were also quantified using global thresholds determined manually by two different approaches. In the validation the manually determined thresholds resulted in large average errors in area fraction (up to 17%), whereas for the evaluation using SEM references, the errors were estimated to be less than 3%. Furthermore, it was found that basing the thresholds on one single SEM reference gave lower errors than determining them manually. This study provides an objective, robust and less error prone method to determine global thresholds for the evaluation of bone formation in CaP scaffolds.
dc.language.isoeng
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Spain
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.subjectÀrees temàtiques de la UPC::Enginyeria biomèdica
dc.subject.lcshBiomedical engineering
dc.subject.otherCalcium phosphate scaffolds
dc.subject.otherMicro-computed tomography
dc.subject.otherBone formation
dc.subject.otherImage analysis
dc.subject.otherSegmentation
dc.titleEvaluation of bone formation in calcium phosphate scaffolds with mu CT-method validation using SEM
dc.typeArticle
dc.subject.lemacEnginyeria biomèdica
dc.contributor.groupUniversitat Politècnica de Catalunya. BAMPLA - Disseny i Avaluació de Xarxes i Serveis de Banda Ampla
dc.contributor.groupUniversitat Politècnica de Catalunya. BBT - Biomaterials, Biomecànica i Enginyeria de Teixits
dc.identifier.doi10.1088/1748-605X/aa801d
dc.description.peerreviewedPeer Reviewed
dc.relation.publisherversionhttp://iopscience.iop.org/article/10.1088/1748-605X/aa801d/meta;jsessionid=71474628864AD2FD87F844F2FD2CBF6A.c1.iopscience.cld.iop.org
dc.rights.accessOpen Access
drac.iddocument21578336
dc.description.versionPostprint (author's final draft)
dc.contributor.covenanteeUniversitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animal
upcommons.citation.authorLewin, S., Barba, A., Persson, C., Franch Serracanta, Jordi, Ginebra, M.P., Ohman, C.
upcommons.citation.publishedtrue
upcommons.citation.publicationNameBiomedical materials
upcommons.citation.volume12
upcommons.citation.number6


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