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dc.contributor.authorAsenova Ivanova, Aleksandra
dc.contributor.authorIvanova, Kristina Dimitrova
dc.contributor.authorHoyo Pérez, Javier
dc.contributor.authorSanchez-Gomez, Susana
dc.contributor.authorHeinze, Thomas
dc.contributor.authorTzanov, Tzanko
dc.contributor.otherUniversitat Politècnica de Catalunya. Departament d'Enginyeria Química
dc.date.accessioned2018-01-24T07:07:37Z
dc.date.available2019-01-09T01:30:45Z
dc.date.issued2018-01-09
dc.identifier.citationAsenova, A., Ivanova, K., Hoyo, J., Sanchez-Gomez, S., Heinze, T., Tzanov, T. Layer-by-layer decorated nanoparticles with tunable antibacterial and antibiofilm properties against both gram-positive and gram-negative bacteria. "ACS applied materials and interfaces", 9 Gener 2018, vol.10, núm.14, p.3314-3323
dc.identifier.issn1944-8244
dc.identifier.urihttp://hdl.handle.net/2117/113123
dc.description.abstractacteria-mediated diseases are a global healthcare concern due to the development and spread of antibiotic resistant strains. Cationic compounds are considered membrane active biocidal agents having a great potential to control bacterial infections, while limiting the emergence of drug resistance. Herein, the versatile and simple Layer-by-Layer (LbL) technique was used to coat alternating multilayers of an antibacterial aminocellulose conjugate and the biocompatible hyaluronic acid on biocompatible polymer nanoparticles (NPs), taking advantage of the nano-size of these otherwise biologically inert templates. Stable polyelectrolyte-decorated particles with an average size of 50 nm and zeta potential of + 40.6 mV were developed after five LbL assembly cycles. The antibacterial activity of these NPs against the Gram-positive Staphylococcus aureus (S. aureus) and Gram-negative Escherichia coli (E. coli) increased significantly when the polycationic aminocellulose was in the outermost layer. The large number of amino groups available on the particles surface, together with the nano-size of the multilayer conjugates, improved their interaction with bacterial membrane phospholipids leading to membrane disruption, as confirmed by a Langmuir monolayer model, and the 10 logs reduction for both bacteria. The biopolymer decorated NPs were also able to inhibit the biofilm formation of S. aureus and E. coli by 94 and 40%, respectively, without affecting human cells viability. The use of LbL coated NPs appears as a promising antibiotic-free alternative for controlling bacterial infections using low amount of antimicrobial agent.
dc.language.isoeng
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Spain
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.subjectÀrees temàtiques de la UPC::Enginyeria química
dc.subject.lcshNanoparticles--Therapeutic use
dc.subject.lcshBiopolymers
dc.subject.lcshBioengineering
dc.subject.otherAminocellulose
dc.subject.otherantibacterial nanoparticles
dc.subject.otherbiofilm prevention
dc.subject.otherhyaluronic acid
dc.subject.otherlayer-by-layer
dc.titleLayer-by-layer decorated nanoparticles with tunable antibacterial and antibiofilm properties against both gram-positive and gram-negative bacteria
dc.typeArticle
dc.subject.lemacNanopartícules -- Ús terapèutic
dc.subject.lemacBiopolímers
dc.subject.lemacBioenginyeria
dc.contributor.groupUniversitat Politècnica de Catalunya. GBMI - Grup de Biotecnologia Molecular i Industrial
dc.identifier.doi10.1021/acsami.7b16508
dc.description.peerreviewedPeer Reviewed
dc.relation.publisherversionhttp://pubs.acs.org/doi/10.1021/acsami.7b16508
dc.rights.accessOpen Access
drac.iddocument21859710
dc.description.versionPostprint (author's final draft)
upcommons.citation.authorAsenova, A.; Ivanova, K.; Hoyo, J.; Sanchez-Gomez, S.; Heinze, T.; Tzanov, T.
upcommons.citation.publishedtrue
upcommons.citation.publicationNameACS applied materials and interfaces
upcommons.citation.volume10
upcommons.citation.number14
upcommons.citation.startingPage3314
upcommons.citation.endingPage3323


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