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dc.contributor.authorZanacchi, Francesca Cella
dc.contributor.authorManzo, Carlo
dc.contributor.authorSandoval Alvarez, Angel
dc.contributor.authorDerr, Nathan D.
dc.contributor.authorGarcía Parajo, María F.
dc.contributor.authorLakadamyali, Melike
dc.contributor.otherUniversitat Politècnica de Catalunya. Institut de Ciències Fotòniques
dc.date.accessioned2017-11-02T16:16:03Z
dc.date.available2017-12-26T01:30:24Z
dc.date.issued2017-06-26
dc.identifier.issn1548-7105
dc.identifier.urihttp://hdl.handle.net/2117/109680
dc.description.abstractSingle-molecule-based super-resolution microscopy offers researchers a unique opportunity to quantify protein copy number with nanoscale resolution. However, while fluorescent proteins have been characterized for quantitative imaging using calibration standards, similar calibration tools for immunofluorescence with small organic fluorophores are lacking. Here we show that DNA origami, in combination with GFP antibodies, is a versatile platform for calibrating fluorophore and antibody labeling efficiency to quantify protein copy number in cellular contexts using super-resolution microscopy.
dc.format.extent7 p.
dc.language.isoeng
dc.publisherNature
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Spain
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.subjectÀrees temàtiques de la UPC::Física
dc.subject.lcshProtein
dc.subject.otherADN
dc.titleDNA Origami offers a versatile method for quantifying protein copy‐number in superresolution
dc.typeArticle
dc.subject.lemacADN
dc.description.peerreviewedPeer Reviewed
dc.relation.publisherversionhttps://www.nature.com/nmeth/journal/v14/n8/full/nmeth.4342.html
dc.rights.accessOpen Access
dc.description.versionPreprint
dc.relation.projectidinfo:eu-repo/grantAgreement/ES/1PE/FIS2015-63550-R
upcommons.citation.publicationNameNATURE METHODS
upcommons.citation.volume14
upcommons.citation.startingPage789
upcommons.citation.endingPage792


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Except where otherwise noted, content on this work is licensed under a Creative Commons license: Attribution-NonCommercial-NoDerivs 3.0 Spain