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dc.contributor.authorSáez Hernández, Alberto
dc.contributor.authorAlmajano Pablos, María Pilar
dc.contributor.authorFernández González, Felipe
dc.contributor.otherUniversitat Politècnica de Catalunya. Departament d'Enginyeria Química
dc.date.accessioned2017-10-25T07:23:11Z
dc.date.available2017-10-25T07:23:11Z
dc.date.issued2017-10-11
dc.identifier.citationSáez, A., Almajano, María Pilar, Fernández, F. A transcriptomic approach to study the effect of long-term starvation and diet composition on the expression of mitochondrial oxidative phosphorylation genes in gilthead sea bream (Sparus aurata). "BMC genomics", 11 Octubre 2017, vol. 18, núm. 768, p. 1-16.
dc.identifier.issn1471-2164
dc.identifier.urihttp://hdl.handle.net/2117/109105
dc.description.abstract© 2017 The Author(s). Background: The impact of nutritional status and diet composition on mitochondrial oxidative phosphorylation (OXPHOS) in fish remains largely unknown. To identify biomarkers of interest in nutritional studies, herein we obtained a deep-coverage transcriptome by 454 pyrosequencing of liver and skeletal muscle cDNA normalised libraries from long-term starved gilthead sea bream (Sparus aurata) and fish fed different diets. Results: After clean-up of high-throughput deep sequencing reads, 699,991 and 555,031 high-quality reads allowed de novo assembly of liver and skeletal muscle sequences, respectively (average length: 374 and 441 bp; total megabases: 262 and 245 Mbp). An additional incremental assembly was completed by integrating data from both tissues (hybrid assembly). Assembly of hybrid, liver and skeletal muscle transcriptomes yielded, respectively, 19,530, 11,545 and 10,599 isotigs (average length: 1330, 1208 and 1390 bp, respectively) that were grouped into 15,954, 10,033 and 9189 isogroups. Following annotation, hybrid transcriptomic data were used to construct an oligonucleotide microarray to analyse nutritional regulation of the expression of 129 genes involved in OXPHOS in S. aurata. Starvation upregulated cytochrome c oxidase components and other key OXPHOS genes in the liver, which exhibited higher sensitive to food deprivation than the skeletal muscle. However, diet composition affected OXPHOS in the skeletal muscle to a greater extent than in the liver: most of genes upregulated under starvation presented higher expression among fish fed a high carbohydrate/low protein diet. Conclusions: Our findings indicate that the expression of coenzyme Q-binding protein (COQ10), cytochrome c oxidase subunit 6A2 (COX6A2) and ADP/ATP translocase 3 (SLC25A6) in the liver, and cytochrome c oxidase subunit 5B isoform 1 (COX5B1) in the liver and the skeletal muscle, are sensitive markers of the nutritional condition that may be relevant to assess the effect of changes in the feeding regime and diet composition on fish farming.
dc.format.extent16 p.
dc.language.isoeng
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Spain
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.subjectÀrees temàtiques de la UPC::Enginyeria química
dc.subject.lcshAntioxidants
dc.subject.lcshNutrition
dc.subject.lcshSparus aurata
dc.subject.otherDiet composition
dc.subject.otherMicroarray
dc.subject.otherOxidative phosphorylation
dc.subject.otherSparus aurata
dc.subject.otherStarvation
dc.subject.otherTranscriptome
dc.titleA transcriptomic approach to study the effect of long-term starvation and diet composition on the expression of mitochondrial oxidative phosphorylation genes in gilthead sea bream (Sparus aurata)
dc.typeArticle
dc.subject.lemacAntioxidants
dc.subject.lemacNutrició
dc.subject.lemacAliments -- Aspectes nutritius
dc.contributor.groupUniversitat Politècnica de Catalunya. ASQUAS - Agricultura Sostenible i Qualitat dels Aliments
dc.identifier.doi10.1186/s12864-017-4148-x
dc.description.peerreviewedPeer Reviewed
dc.relation.publisherversionhttps://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-017-4148-x
dc.rights.accessOpen Access
drac.iddocument21578246
dc.description.versionPostprint (published version)
upcommons.citation.authorSáez, A., Almajano, María Pilar, Fernández, F.
upcommons.citation.publishedtrue
upcommons.citation.publicationNameBMC genomics
upcommons.citation.volume18
upcommons.citation.number768
upcommons.citation.startingPage1
upcommons.citation.endingPage16
dc.identifier.pmid29020939


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Except where otherwise noted, content on this work is licensed under a Creative Commons license: Attribution-NonCommercial-NoDerivs 3.0 Spain