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Real-time bioluminescence imaging of cell distribution, growth, and differentiation in a three-dimensional scaffold under interstitial perfusion for tissue engineering
dc.contributor.author | Vila, Olaia |
dc.contributor.author | Garrido, Cristina |
dc.contributor.author | Guerra Rebollo, Marta |
dc.contributor.author | Navarro Toro, Melba |
dc.contributor.author | Meca-Cortes, O |
dc.contributor.author | Mas, S |
dc.contributor.author | Engel López, Elisabeth |
dc.contributor.author | Rubio, Nuria |
dc.contributor.author | Blanco, Jeronimo |
dc.contributor.other | Universitat Politècnica de Catalunya. Departament de Ciència dels Materials i Enginyeria Metal·lúrgica |
dc.date.accessioned | 2017-06-16T13:16:14Z |
dc.date.issued | 2016-09-01 |
dc.identifier.citation | Vila, O., Garrido, C., Guerra, M., Navarro, M., Meca-Cortes, O., Mas, S., Engel, E., Rubio, N., Blanco, J. Real-time bioluminescence imaging of cell distribution, growth, and differentiation in a three-dimensional scaffold under interstitial perfusion for tissue engineering. "Tissue engineering. Part C, methods", 1 Setembre 2016, vol. 22, núm. 9, p. 864-872. |
dc.identifier.issn | 1937-3384 |
dc.identifier.uri | http://hdl.handle.net/2117/105536 |
dc.description.abstract | Bioreactor systems allow safe and reproducible production of tissue constructs and functional analysis of cell behavior in biomaterials. However, current procedures for the analysis of tissue generated in biomaterials are destructive. We describe a transparent perfusion system that allows real-time bioluminescence imaging of luciferase expressing cells seeded in scaffolds for the study of cell–biomaterial interactions and bioreactor performance. A prototype provided with a poly(lactic) acid scaffold was used for ‘‘proof of principle’’ studies to monitor cell survival in the scaffold (up to 22 days). Moreover, using cells expressing a luciferase reporter under the control of inducible tissue-specific promoters, it was possible to monitor changes in gene expression resulting from hypoxic state and endothelial cell differentiation. This system should be useful in numerous tissue engineering applications, the optimization of bioreactor operation conditions, and the analysis of cell behavior in three-dimensional scaffolds. |
dc.format.extent | 9 p. |
dc.language.iso | eng |
dc.subject | Àrees temàtiques de la UPC::Enginyeria dels materials |
dc.subject.lcsh | Bioluminescence |
dc.subject.lcsh | Tissue engineering |
dc.title | Real-time bioluminescence imaging of cell distribution, growth, and differentiation in a three-dimensional scaffold under interstitial perfusion for tissue engineering |
dc.type | Article |
dc.subject.lemac | Enginyeria de teixits |
dc.subject.lemac | Luminescència |
dc.contributor.group | Universitat Politècnica de Catalunya. BBT - Biomaterials, Biomecànica i Enginyeria de Teixits |
dc.identifier.doi | 10.1089/ten.tec.2014.0421 |
dc.description.peerreviewed | Peer Reviewed |
dc.rights.access | Restricted access - publisher's policy |
local.identifier.drac | 21094214 |
dc.description.version | Postprint (published version) |
dc.date.lift | 10000-01-01 |
local.citation.author | Vila, O.; Garrido, C.; Guerra, M.; Navarro, M.; Meca-Cortes, O.; Mas, S.; Engel, E.; Rubio, N.; Blanco, J. |
local.citation.publicationName | Tissue engineering. Part C, methods |
local.citation.volume | 22 |
local.citation.number | 9 |
local.citation.startingPage | 864 |
local.citation.endingPage | 872 |
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