Sphingomyelin metabolism controls the shape and function of the Golgi cisternae
Tipus de documentArticle
EditoreLife Sciences Publications
Condicions d'accésAccés obert
Projecte de la Comissió EuropeaNANO-VISTA - Advanced photonic antenna tools for biosensing and cellular nanoimaging (EC-FP7-288263)
MUPS - Mechanism of Unconventional Protein Secretion (EC-FP7-268692)
The flat Golgi cisterna is a highly conserved feature of eukaryotic cells, but how is this morphology achieved and is it related to its function in cargo sorting and export? A physical model of cisterna morphology led us to propose that sphingomyelin (SM) metabolism at the trans-Golgi membranes in mammalian cells essentially controls the structural features of a Golgi cisterna by regulating its association to curvature-generating proteins. An experimental test of this hypothesis revealed that affecting SM homeostasis converted flat cisternae into highly curled membranes with a concomitant dissociation of membrane curvature-generating proteins. These data lend support to our hypothesis that SM metabolism controls the structural organization of a Golgi cisterna. Together with our previously presented role of SM in controlling the location of proteins involved in glycosylation and vesicle formation, our data reveal the significance of SM metabolism in the structural organization and function of Golgi cisternae.
CitacióCampelo, F. [et al.]. Sphingomyelin metabolism controls the shape and function of the Golgi cisternae. "eLife", 13 Maig 2017.
Versió de l'editorhttps://elifesciences.org/content/6/e24603