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dc.contributor.authorCiapetti, Gabriela
dc.contributor.authorPompo, Gemma di
dc.contributor.authorAvnet, Sofia
dc.contributor.authorMartini, Desirée
dc.contributor.authorDíez Escudero, Anna
dc.contributor.authorMontufar Jiménez, Edgar Benjamin
dc.contributor.authorGinebra Molins, Maria Pau
dc.contributor.authorBaldini, Nicola
dc.contributor.otherUniversitat Politècnica de Catalunya. Departament de Ciència dels Materials i Enginyeria Metal·lúrgica
dc.identifier.citationCiapetti, G., Pompo, G., Avnet, S., Martini, D., Diez, A., Montufar, Edgar B., Ginebra, M.P., Baldini, N. Osteoclast differentiation from human blood precursors on biomimetic calcium-phosphate substrates. "Acta biomaterialia", 1 Març 2017, vol. 50, p. 102-113.
dc.description.abstractThe design of synthetic bone grafts to foster bone formation is a challenge in regenerative medicine. Understanding the interaction of bone substitutes with osteoclasts is essential, since osteoclasts not only drive a timely resorption of the biomaterial, but also trigger osteoblast activity. In this study, the adhesion and differentiation of human blood-derived osteoclast precursors (OCP) on two different micro-nanostructured biomimetic hydroxyapatite materials consisting in coarse (HA-C) and fine HA (HA-F) crystals, in comparison with sintered stoichiometric HA (sin-HA, reference material), were investigated. Osteoclasts were induced to differentiate by RANKL-containing supernatant using cell/substrate direct and indirect contact systems, and calcium (Ca++) and phosphorus (P5+) in culture medium were measured. We observed that OCP adhered to the experimental surfaces, and that osteoclast-like cells formed at a rate influenced by the micro- and nano-structure of HA, which also modulate extracellular Ca++. Qualitative differences were found between OCP on biomimetic HA-C and HA-F and their counterparts on plastic and sin-HA. On HA-C and HA-F cells shared typical features of mature osteoclasts, i.e. podosomes, multinuclearity, tartrate acid phosphatase (TRAP)-positive staining, and TRAP5b-enzyme release. However, cells were less in number compared to those on plastic or on sin-HA, and they did not express some specific osteoclast markers. In conclusion, blood-derived OCP are able to attach to biomimetic and sintered HA substrates, but their subsequent fusion and resorptive activity are hampered by surface micro-nano-structure. Indirect cultures suggest that fusion of OCP is sensitive to topography and to extracellular calcium.
dc.format.extent12 p.
dc.subjectÀrees temàtiques de la UPC::Enginyeria dels materials
dc.subject.lcshBone resorption
dc.subject.otherIonic exchange
dc.subject.otherBone resorption
dc.titleOsteoclast differentiation from human blood precursors on biomimetic calcium-phosphate substrates
dc.contributor.groupUniversitat Politècnica de Catalunya. BBT - Biomaterials, Biomecànica i Enginyeria de Teixits
dc.rights.accessOpen Access
dc.contributor.covenanteeIstituto Ortopedico Rizzoli
local.citation.authorCiapetti, G.; Pompo, G.; Avnet, S.; Martini, D.; Diez, A.; Montufar, Edgar B.; Ginebra, M.P.; Baldini, N.
local.citation.publicationNameActa biomaterialia

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