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Cell adhesive peptides functionalized on CoCr alloy stimulate endothelialization and prevent thrombogenesis and restenosis
dc.contributor.author | Castellanos, Maria Isabel |
dc.contributor.author | Guillem Martí, Jordi |
dc.contributor.author | Mas Moruno, Carlos |
dc.contributor.author | Díaz Ricart, Maribel |
dc.contributor.author | Escolar Albaladejo, Ginés |
dc.contributor.author | Ginebra Molins, Maria Pau |
dc.contributor.author | Gil Mur, Francisco Javier |
dc.contributor.author | Pegueroles Neyra, Marta |
dc.contributor.author | Manero Planella, José María |
dc.contributor.other | Universitat Politècnica de Catalunya. Departament de Ciència dels Materials i Enginyeria Metal·lúrgica |
dc.date.accessioned | 2017-05-05T16:45:39Z |
dc.date.available | 2018-04-03T00:30:21Z |
dc.date.issued | 2017-04-01 |
dc.identifier.citation | Castellanos, M., Guillem-Marti, J., Mas-Moruno, C., Díaz-Ricart, M., Escolar, G., Ginebra, M.P., Gil, J., Pegueroles, Marta, Manero, J. Cell adhesive peptides functionalized on CoCr alloy stimulate endothelialization and prevent thrombogenesis and restenosis. "Journal of biomedical materials research. Part A", 1 Abril 2017, vol. 105, núm. 4, p. 973-983. |
dc.identifier.issn | 1549-3296 |
dc.identifier.uri | http://hdl.handle.net/2117/104138 |
dc.description.abstract | Immobilization of bioactive peptide sequences on CoCr surfaces is an effective route to improve endothelialization, which is of great interest for cardiovascular stents. In this work, we explored the effect of physical and covalent immoblization of RGDS, YIGSR and their equimolar combination peptides on endothelial cells (EC) and smooth muscle cell (SMC) adhesion and on thrombogenicity. We extensively investigated using RT-qPCR, the expression by ECs cultured on functionalised CoCr surfaces of different genes. Genes relevant for adhesion (ICAM-1 and VCAM-1), vascularization (VEGFA, VEGFR-1 and VEGFR-2) and anti-thrombogenicity (tPA and eNOS) were over-expressed in the ECs grown to covalently functionalized CoCr surfaces compared to physisorbed and control surfaces. Pro-thrombogenic genes expression (PAI-1 and vWF) decreased over time. Cell co-cultures of ECs/SMCs found that functionalization increased the amount of adhered ECs onto modified surfaces compared to plain CoCr, independently of the used peptide and the strategy of immobilization. SMCs adhered less compared to ECs in all surfaces. All studied peptides showed a lower platelet cell adhesion compared to TCPS. Covalent functionalization of CoCr surfaces with an equimolar combination of RGDS and YIGSR represented prevailing strategy to enhance the early stages of ECs adhesion and proliferation, while preventing SMCs and platelet adhesion. |
dc.format.extent | 11 p. |
dc.language.iso | eng |
dc.subject | Àrees temàtiques de la UPC::Enginyeria dels materials |
dc.subject.lcsh | Gene expression |
dc.subject.other | functionalization |
dc.subject.other | CoCr alloy |
dc.subject.other | gene expression |
dc.subject.other | platelet adhesion |
dc.subject.other | cell coculture |
dc.title | Cell adhesive peptides functionalized on CoCr alloy stimulate endothelialization and prevent thrombogenesis and restenosis |
dc.type | Article |
dc.subject.lemac | Expressió gènica |
dc.contributor.group | Universitat Politècnica de Catalunya. BBT - Biomaterials, Biomecànica i Enginyeria de Teixits |
dc.identifier.doi | 10.1002/jbm.a.35988 |
dc.relation.publisherversion | http://onlinelibrary.wiley.com/doi/10.1002/jbm.a.35988/abstract |
dc.rights.access | Open Access |
local.identifier.drac | 20329846 |
dc.description.version | Postprint (author's final draft) |
dc.contributor.covenantee | Hospital Clínic i Provincial de Barcelona |
local.citation.author | Castellanos, M.; Guillem-Marti, J.; Mas-Moruno, C.; Díaz-Ricart, M.; Escolar, G.; Ginebra, M.P.; Gil, J.; Pegueroles, Marta; Manero, J. |
local.citation.publicationName | Journal of biomedical materials research. Part A |
local.citation.volume | 105 |
local.citation.number | 4 |
local.citation.startingPage | 973 |
local.citation.endingPage | 983 |
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