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dc.contributor.authorDíez Escudero, Anna
dc.contributor.authorEspañol Pons, Montserrat
dc.contributor.authorMontufar Jiménez, Edgar Benjamin
dc.contributor.authorPompo, Gemma di
dc.contributor.authorCiapetti, Gabriela
dc.contributor.authorBaldini, Nicola
dc.contributor.authorGinebra Molins, Maria Pau
dc.contributor.otherUniversitat Politècnica de Catalunya. Departament de Ciència dels Materials i Enginyeria Metal·lúrgica
dc.identifier.citationDiez, A., Español, M., Montufar, Edgar B., Pompo, G., Ciapetti, G., Baldini, N., Ginebra, M.P. Focus ion beam/scanning electron microscopy characterization of osteoclastic resorption of calcium phosphate substrates. "Tissue engineering. Part C, methods", 1 Febrer 2017, vol. 23, núm. 2, p. 118-124.
dc.description.abstractThis article presents the application of dual focused ion beam/scanning electron microscopy (FIB-SEM) imaging for preclinical testing of calcium phosphates with osteoclast precursor cells and how this high-resolution imaging technique is able to reveal microstructural changes at a level of detail previously not possible. Calcium phosphate substrates, having similar compositions but different microstructures, were produced using low- and high-temperature processes (biomimetic calcium-deficient hydroxyapatite [CDHA] and stoichiometric sintered hydroxyapatite, respectively). Human osteoclast precursor cells were cultured for 21 days before evaluating their resorptive potential on varying microstructural features. Alternative to classical morphological evaluation of osteoclasts (OC), FIB-SEM was used to observe the subjacent microstructure by transversally sectioning cells and observing both the cells and the substrates. Resorption pits, indicating OC activity, were visible on the smoother surface of high-temperature sintered hydroxyapatite. FIB-SEM analysis revealed signs of acidic degradation on the grain surface under the cells, as well as intergranular dissolution. No resorption pits were evident on the surface of the rough CDHA substrates. However, whereas no degradation was detected by FIB sections in the material underlying some of the cells, early stages of OC-mediated acidic degradation were observed under cells with more spread morphology. Collectively, these results highlight the potential of FIB to evaluate the resorptive activity of OC, even in rough, irregular, or coarse surfaces where degradation pits are otherwise difficult to visualize.
dc.format.extent7 p.
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Spain
dc.subjectÀrees temàtiques de la UPC::Enginyeria dels materials
dc.subject.lcshCalcium Phosphates
dc.subject.lcshBiomedical materials
dc.subject.lcshTissue engineering
dc.titleFocus ion beam/scanning electron microscopy characterization of osteoclastic resorption of calcium phosphate substrates
dc.subject.lemacFosfat de calci
dc.subject.lemacMaterials biomèdics
dc.subject.lemacEnginyeria de teixits
dc.contributor.groupUniversitat Politècnica de Catalunya. BBT - Biomaterials, Biomecànica i Enginyeria de Teixits
dc.description.peerreviewedPeer Reviewed
dc.rights.accessOpen Access
dc.description.versionPostprint (author's final draft)
dc.contributor.covenanteeIstituto Ortopedico Rizzoli
local.citation.authorDiez, A.; Español, M.; Montufar, Edgar B.; Pompo, G.; Ciapetti, G.; Baldini, N.; Ginebra, M.P.
local.citation.publicationNameTissue engineering. Part C, methods

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