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dc.contributor.authorIvanova, Kristina Dimitrova
dc.contributor.authorHoyo Pérez, Javier
dc.contributor.authorFrancesko, Antonio
dc.contributor.authorTzanov, Tzanko
dc.contributor.authorFernandes, Margarida M.
dc.contributor.authorPérez Rafael, Silvia
dc.contributor.otherUniversitat Politècnica de Catalunya. Departament d'Enginyeria Química
dc.identifier.citationFernandes, M.M., Ivanova, K., Hoyo, J., Perez-Rafael, S., Francesko, A., Tzanov, T. Nanotransformation of vancomycin overcomes the intrinsic resistance of Gram-negative bacteria. "ACS applied materials and interfaces", 10 Abril 2017.
dc.description.abstractThe increased emergence of antibiotic-resistant bacteria is a growing public health concern, and although new drugs are constantly being sought, the pace of development is slow compared with the evolution and spread of multidrug- resistant species. In this study, we developed a novel broad-spectrum antimicrobial agent by simply transforming vancomycin into nanoform using sonochemistry. Vancomycin is a glycopeptide antibiotic largely used for the treatment of infections caused by Gram-positive bacteria but inefficient against Gram-negative species. The nanospherization extended its effect toward Gram-negative Escherichia coli and Pseudomonas aeruginosa, making these bacteria up to 10 and 100 times more sensitive to the antibiotic, respectively. The spheres were able to disrupt the outer membranes of these bacteria, overcoming their intrinsic resistance toward glycopeptides. The penetration of nanospheres into a Langmuir monolayer of bacterial membrane phospholipids confirmed the interaction of the nanoantibiotic with the membrane of E. coli cells, affecting their physical integrity, as further visualized by scanning electron microscopy. Such mechanism of antibacterial action is unlikely to induce mutations in the evolutionary conserved bacterial membrane, therefore reducing the possibility of acquiring resistance. Our results indicated that the nanotransformation of vancomycin could overcome the inherent resistance of Gram-negative bacteria toward this antibiotic and disrupt mature biofilms at antibacterial-effective concentrations.
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Spain
dc.subjectÀrees temàtiques de la UPC::Enginyeria química
dc.subject.otherantibiotic nanospheres
dc.subject.otherantibiotic resistance
dc.titleNanotransformation of vancomycin overcomes the intrinsic resistance of Gram-negative bacteria
dc.contributor.groupUniversitat Politècnica de Catalunya. GBMI - Grup de Biotecnologia Molecular i Industrial
dc.description.peerreviewedPeer Reviewed
dc.rights.accessOpen Access
dc.description.versionPostprint (author's final draft)
local.citation.authorFernandes, M.M.; Ivanova, K.; Hoyo, J.; Francesko, A.; Tzanov, T.
local.citation.publicationNameACS applied materials and interfaces

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