Diarrhoea caused by rotavirus in a regional Peruvian hospital: Determination of circulating genotypes
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Background: Gastroenteritis caused by rotavirus is responsible for approximately 810 deaths per year in children under 5 years of age in Peru and emerging rotavirus genotypes have led to concerns regarding cross-protection by the vaccines available. Moreover, there are no reports on the molecular epidemiology of rotavirus diarrhoea in Peru. Methods: A total of 131 stool samples were obtained from children under 5 years hospitalised from January 2010 to December 2012 in the Hospital Regional de Cajamarca (Peru). ELISA and RT-PCR techniques were performed for rotavirus detection. G and P typing of rotavirus-positive samples were performed by semi-nested multiplex RT-PCR, and sequencing was performed to confirm the PCR results. Results: Of the 117 samples available, 22 (18.8%) tested positive for rotavirus by ELISA and 42 (35.9%) tested positive by RT-PCR. Among the G genotypes identified, G9 (35.7%; 15/42) and G12 (33.3%; 14/42) were the most prevalent, with the most common combination being G12/P (23.8%; 10/42). Conclusions: A high prevalence of the G12/P genotype was detected. It is known that this genotype is not covered by the current vaccines available. More in-depth studies are needed to determine the current rotavirus genotypes presents in Peru. © The Author 2014. Published by Oxford University Press on behalf of Royal Society of Tropical Medicine and Hygiene. All rights reserved.
CitationEspejo, P., Peralta, F., Cornejo, H., del Valle, LJ., Tapia, A., Bazan, J., Ruiz, J., Del Valle, J. Diarrhoea caused by rotavirus in a regional Peruvian hospital: Determination of circulating genotypes. "Transactions of the Royal Society of Tropical Medicine and Hygiene", Juliol 2014, vol. 108, núm. 7, p. 425-430.