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dc.contributor.authorSrinivasan, Sundaramoorthy
dc.contributor.authorCordomí, Arnau
dc.contributor.authorRamon Portés, Eva
dc.contributor.authorGarriga Solé, Pere
dc.contributor.otherUniversitat Politècnica de Catalunya. Departament d'Enginyeria Química
dc.date.accessioned2017-03-20T12:13:10Z
dc.date.available2017-03-20T12:13:10Z
dc.date.issued2016-03
dc.identifier.citationSrinivasan, S., Cordomí, A., Ramon, E., Garriga, P. Beyond spectral tuning: human cone visual pigments adopt different transient conformations for chromophore regeneration. "Cellular and molecular life sciences", Març 2016, vol. 73, núm. 6, p. 1253-1263.
dc.identifier.issn1420-682X
dc.identifier.urihttp://hdl.handle.net/2117/102660
dc.descriptionThe final publication is available at Springer via http://dx.doi.org/10.1007/s00018-015-2043-7
dc.description.abstractHuman red and green visual pigments are seven transmembrane receptors of cone photoreceptor cells of the retina that mediate color vision. These pigments share a very high degree of homology and have been assumed to feature analogous structural and functional properties. We report on a different regeneration mechanism among red and green cone opsins with retinal analogs using UV–Vis/fluorescence spectroscopic analyses, molecular modeling and site-directed mutagenesis. We find that photoactivated green cone opsin adopts a transient conformation which regenerates via an unprotonated Schiff base linkage with its natural chromophore, whereas red cone opsin forms a typical protonated Schiff base. The chromophore regeneration kinetics is consistent with a secondary retinal uptake by the cone pigments. Overall, our findings reveal, for the first time, structural differences in the photoactivated conformation between red and green cone pigments that may be linked to their molecular evolution, and support the proposal of secondary retinal binding to visual pigments, in addition to binding to the canonical primary site, which may serve as a regulatory mechanism of dark adaptation in the phototransduction process.
dc.format.extent11 p.
dc.language.isoeng
dc.subjectÀrees temàtiques de la UPC::Ciències de la visió::Biologia ocular
dc.subject.lcshG proteins -- Receptors
dc.subject.lcshColor vision
dc.subject.otherColor vision
dc.subject.otherLigand binding
dc.subject.otherVisual phototransduction
dc.subject.otherRetinal
dc.subject.otherG-protein coupled
dc.subject.otherReceptors
dc.titleBeyond spectral tuning: human cone visual pigments adopt different transient conformations for chromophore regeneration
dc.typeArticle
dc.subject.lemacProteïnes G -- Receptors
dc.subject.lemacSentit del color
dc.contributor.groupUniversitat Politècnica de Catalunya. GBMI - Grup de Biotecnologia Molecular i Industrial
dc.identifier.doi10.1007/s00018-015-2043-7
dc.description.peerreviewedPeer Reviewed
dc.rights.accessOpen Access
drac.iddocument19701419
dc.description.versionPostprint (author's final draft)
upcommons.citation.authorSrinivasan, S., Cordomí, A., Ramon, E., Garriga, P.
upcommons.citation.publishedtrue
upcommons.citation.publicationNameCellular and molecular life sciences
upcommons.citation.volume73
upcommons.citation.number6
upcommons.citation.startingPage1253
upcommons.citation.endingPage1263


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