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Third-harmonic generation for the study of Caenorhabditis elegans embryogenesis
dc.contributor.author | Aviles Espinosa, Rodrigo |
dc.contributor.author | Santos, Susana I.C.O. |
dc.contributor.author | Brodschelm, Andreas |
dc.contributor.author | Kaenders, Wilhelm G. |
dc.contributor.author | Alonso Ortega, Cesar |
dc.contributor.author | Artigas García, David |
dc.contributor.author | Loza Álvarez, Pablo |
dc.contributor.other | Universitat Politècnica de Catalunya. Departament de Teoria del Senyal i Comunicacions |
dc.date.accessioned | 2010-11-12T10:21:48Z |
dc.date.available | 2010-11-12T10:21:48Z |
dc.date.created | 2010-08 |
dc.date.issued | 2010-08 |
dc.identifier.citation | Aviles, R. [et al.]. Third-harmonic generation for the study of Caenorhabditis elegans embryogenesis. "Journal of biomedical optics. SPIE, International Society for Optical Engineering", Agost 2010, vol. 15, núm. 4, p. 046020-1-046020-7. |
dc.identifier.issn | 1083-3668 |
dc.identifier.uri | http://hdl.handle.net/2117/10263 |
dc.description.abstract | Live microscopy techniques i.e., differential interference contrast, confocal microscopy, etc. have enabled the understanding of the mechanisms involved in cells and tissue formation. In long-term studies, special care must be taken in order to avoid sample damage, restricting the applicability of the different microscopy techniques.We demonstrate the potential of using third-harmonic generation THG microscopy for morphogenesis/embryogenesis studies in living Caenorhabditis elegans C. elegans . Moreover, we show that the THG signal is obtained in all the embryo development stages, showing different tissue/structure information. For this research, we employ a 1550-nm femtosecond fiber laser and demonstrate that the expected water absorption at this wavelength does not severely compromise sample viability. Additionally, this has the important advantage that the THG signal is emitted at visible wavelengths 516 nm . Therefore, standard collection optics and detectors operating near maximum efficiency enable an optimal signal reconstruction. All this, to the best of our knowledge, demonstrates for the first time the noninvasiveness and strong potential of this particular wavelength to be used for highresolution four-dimensional imaging of embryogenesis using unstained C. elegans in vivo samples. |
dc.language.iso | eng |
dc.subject.lcsh | Multiphoton processes |
dc.subject.lcsh | Fiber optic cables |
dc.subject.lcsh | Imaging systems in biology |
dc.subject.lcsh | Signal theory (Telecommunication) |
dc.title | Third-harmonic generation for the study of Caenorhabditis elegans embryogenesis |
dc.type | Article |
dc.subject.lemac | Senyal, Teoria del (Telecomunicació) |
dc.contributor.group | Universitat Politècnica de Catalunya. FOTONICA - Grup de Recerca de Fotònica |
dc.identifier.doi | 10.1117/1.3477535 |
dc.relation.publisherversion | http://link.aip.org/getpdf/servlet/GetPDFServlet?filetype=pdf&id=JBOPFO000015000004046020000001 |
dc.rights.access | Open Access |
local.identifier.drac | 2811772 |
dc.description.version | Postprint (published version) |
dc.relation.projectid | info:eu-repo/grantAgreement/EC/FP7/217997/EU/Spatio-Temporal Engineering of Light. Ultimate Multiphoton Microscopy/STELUM |
dc.relation.projectid | info:eu-repo/grantAgreement/EC/FP7/224014/EU/Network of Excellence for Biophotonics/PHOTONICS4LIFE |
dc.relation.projectid | info:eu-repo/grantAgreement/EC/FP7/224338/EU/COMPACT ULTRAFAST LASER SOURCES BASED ON NOVEL QUANTUM DOT STRUCTURES/FAST-DOT |
local.citation.author | Aviles, R.; Santos, S.; Brodschelm, A.; Kaenders, W.; Alonso, C.; Artigas, D.; Loza, P. |
local.citation.publicationName | Journal of biomedical optics. SPIE, International Society for Optical Engineering |
local.citation.volume | 15 |
local.citation.number | 4 |
local.citation.startingPage | 046020-1 |
local.citation.endingPage | 046020-7 |
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