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dc.contributor.authorValle Mendoza, Luis Javier del
dc.contributor.authorJaramillo, Michael L.
dc.contributor.authorTalledo, Miguel
dc.contributor.authorPons, Maria J.
dc.contributor.authorFlores, Lídia
dc.contributor.authorQuispe, Ruth
dc.contributor.authorRamírez, Pablo
dc.contributor.authorGarcía de la Guarda, Ruth
dc.contributor.authorAlvarado, Débora
dc.contributor.authorEspinoza Culupú, Abraham
dc.contributor.authorValle Mendoza, Juana del
dc.contributor.authorVargas, Martha
dc.contributor.authorRuiz, Joaquim
dc.contributor.otherUniversitat Politècnica de Catalunya. Departament d'Enginyeria Química
dc.date.accessioned2017-03-16T11:01:40Z
dc.date.issued2014
dc.identifier.citationdel Valle, LJ., Jaramillo, M., Talledo, M., Pons, M., Flores, L., Quispe, R., Ramírez, P., García, R., Alvarado, D., Espinoza, A., Del Valle, J., Vargas, M., Ruiz, J. Development of a 16S rRNA PCR-RFLP Assay for Bartonella identification: Applicability in the identification of species involved in human infections. "Universal Journal of Microbiology Research", 2014, vol. 2, núm. 1, p. 15-22.
dc.identifier.issn2331-6721
dc.identifier.urihttp://hdl.handle.net/2117/102572
dc.description.abstractWe designed a 16S rRNA gene PCR -RFLP scheme to identify all currently described Bartonella spp. The 16S rRNA genes of all Bartonella spp. were in-silico analyzed in order to design a RFLP technique able to discriminate among different species. The restriction enzymes selected were Mae III, Mse I, Sau96 I, Bsa AI, Drd I, Fok I, Bss HII, Bst UI, Alu I, Tsp DTI and Hph I which, according to a decision -making tree, faci litated the differentiation of all the currently described species of Bartonella .The technique was experimentally tested in different species of Bartonella , including human pathogenic B. bacilliformis and B. henselae with a 100% of concordance with the in-silico predicted patterns.This novel RFLP assay could be used to identify both human and non- human pathogenic Bartonella in diagnostic, phylogenetic and epidemiologic studies
dc.format.extent8 p.
dc.language.isoeng
dc.publisherSociety of Exploration Geophysicists (SEG)
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.subjectÀrees temàtiques de la UPC::Enginyeria química
dc.subject.lcshBartonella
dc.subject.lcshGenes
dc.subject.otherBartonella
dc.subject.otherPCR-RFLP
dc.subject.other16s rRNA Gene
dc.subject.otherIdentification
dc.titleDevelopment of a 16S rRNA PCR-RFLP Assay for Bartonella identification: Applicability in the identification of species involved in human infections
dc.typeArticle
dc.subject.lemacGens
dc.contributor.groupUniversitat Politècnica de Catalunya. PSEP - Polimers Sintètics: Estructura i Propietats. Polimers Biodegradables
dc.identifier.doi10.13189/ujmr.2014.020102
dc.description.peerreviewedPeer Reviewed
dc.relation.publisherversionwww.hrpub.org
dc.rights.accessRestricted access - publisher's policy
local.identifier.drac19789908
dc.description.versionPostprint (author's final draft)
dc.date.lift10000-01-01
local.citation.authordel Valle, LJ.; Jaramillo, M.; Talledo, M.; Pons, M.; Flores, L.; Quispe, R.; Ramírez, P.; García, R.; Alvarado, D.; Espinoza, A.; Del Valle, J.; Vargas, M.; Ruiz, J.
local.citation.publicationNameUniversal Journal of Microbiology Research
local.citation.volume2
local.citation.number1
local.citation.startingPage15
local.citation.endingPage22


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