Show simple item record

dc.contributor.authorFernandez-Sampedro, Miguel angel
dc.contributor.authorInvergo, Brandon M.
dc.contributor.authorRamon Portés, Eva
dc.contributor.authorBertranpetit, Jaume
dc.contributor.authorGarriga Solé, Pere
dc.contributor.otherUniversitat Politècnica de Catalunya. Departament d'Enginyeria Química
dc.date.accessioned2017-03-10T09:40:05Z
dc.date.available2017-03-10T09:40:05Z
dc.date.issued2016-02-11
dc.identifier.citationFernandez-Sampedro, M., Invergo, B., Ramon, E., Bertranpetit, J., Garriga, P. Functional role of positively selected amino acid substitutions in mammalian rhodopsin evolution. "Scientific reports", 11 Febrer 2016, vol. 6, núm. 21570.
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/2117/102267
dc.description.abstractVisual rhodopsins are membrane proteins that function as light photoreceptors in the vertebrate retina. Specific amino acids have been positively selected in visual pigments during mammal evolution, which, as products of adaptive selection, would be at the base of important functional innovations. We have analyzed the top candidates for positive selection at the specific amino acids and the corresponding reverse changes (F13M, Q225R and A346S) in order to unravel the structural and functional consequences of these important sites in rhodopsin evolution. We have constructed, expressed and immunopurified the corresponding mutated pigments and analyzed their molecular phenotypes. We find that position 13 is very important for the folding of the receptor and also for proper protein glycosylation. Position 225 appears to be important for the function of the protein affecting the G-protein activation process, and position 346 would also regulate functionality of the receptor by enhancing G-protein activation and presumably affecting protein phosphorylation by rhodopsin kinase. Our results represent a link between the evolutionary analysis, which pinpoints the specific amino acid positions in the adaptive process, and the structural and functional analysis, closer to the phenotype, making biochemical sense of specific selected genetic sequences in rhodopsin evolution.
dc.language.isoeng
dc.publisherMacmillan Publishers
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/es/
dc.subjectÀrees temàtiques de la UPC::Ciències de la salut::Medicina
dc.subject.lcshG proteins--Receptors
dc.subject.otherDOMINANT RETINITIS-PIGMENTOSA
dc.subject.otherVERTEBRATE VISUAL PIGMENTS
dc.subject.otherDIM-LIGHT VISION
dc.subject.otherRETINAL DEGENERATION
dc.subject.otherMOLECULAR EVOLUTION
dc.subject.otherBOVINE RHODOPSIN
dc.subject.otherFLUORESCENCE SPECTROSCOPY
dc.subject.otherTRANSDUCIN ACTIVATION
dc.subject.otherLINKED GLYCOSYLATION
dc.subject.otherMAXIMUM-LIKELIHOOD
dc.titleFunctional role of positively selected amino acid substitutions in mammalian rhodopsin evolution
dc.typeArticle
dc.subject.lemacProteïnes G -- Receptors
dc.contributor.groupUniversitat Politècnica de Catalunya. GBMI - Grup de Biotecnologia Molecular i Industrial
dc.identifier.doi10.1038/srep21570
dc.description.peerreviewedPeer Reviewed
dc.relation.publisherversionhttp://www.nature.com/articles/srep21570
dc.rights.accessOpen Access
local.identifier.drac19772054
dc.description.versionPostprint (published version)
local.citation.authorFernandez-Sampedro, M.; Invergo, B.; Ramon, E.; Bertranpetit, J.; Garriga, P.
local.citation.publicationNameScientific reports
local.citation.volume6
local.citation.number21570
dc.identifier.pmid26865329


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record

Attribution 3.0 Spain
Except where otherwise noted, content on this work is licensed under a Creative Commons license : Attribution 3.0 Spain