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Functional protein-based nanomaterial produced in microorganisms recognized as safe: A new platform for biotechnology
dc.contributor.author | Cano Garrido, Olivia |
dc.contributor.author | Sanchez Chardi, Alejandro |
dc.contributor.author | Parés, Silvia |
dc.contributor.author | Giró, Irene |
dc.contributor.author | Tatkiewicz, Witold I. |
dc.contributor.author | Ferrer Miralles, Neus |
dc.contributor.author | Ratera, Imma |
dc.contributor.author | Natalello, Antonino |
dc.contributor.author | Cubarsí Morera, Rafael |
dc.contributor.author | Veciana Miró, Jaume |
dc.contributor.author | Bach, Alex |
dc.contributor.author | Villaverde Corrales, Antonio Pedro |
dc.contributor.author | Arís, Anna |
dc.contributor.author | García Fruitós, Elena |
dc.contributor.other | Universitat Politècnica de Catalunya. Departament de Matemàtiques |
dc.date.accessioned | 2017-03-09T09:45:06Z |
dc.date.available | 2018-10-02T00:30:30Z |
dc.date.issued | 2016-10-01 |
dc.identifier.citation | Cano, O., Sanchez, A., Parés, S., Giró, I., Tatkiewicz, W., Ferrer-Miralles, N., Ratera, I., Natalello, A., Cubarsi, R., Veciana, J., Bach, A., Villaverde, A., Aris, A., García, E. Functional protein-based nanomaterial produced in microorganisms recognized as safe: A new platform for biotechnology. "Acta biomaterialia", 1 Octubre 2016, vol. 43, p. 230-239. |
dc.identifier.issn | 1742-7061 |
dc.identifier.uri | http://hdl.handle.net/2117/102175 |
dc.description.abstract | Inclusion bodies (IBs) are protein-based nanoparticles formed in Escherichia coli through stereospecific aggregation processes during the overexpression of recombinant proteins. In the last years, it has been shown that IBs can be used as nanostructured biomaterials to stimulate mammalian cell attachment, proliferation, and differentiation. In addition, these nanoparticles have also been explored as natural delivery systems for protein replacement therapies. Although the production of these protein-based nanomaterials in E. coli is economically viable, important safety concerns related to the presence of endotoxins in the products derived from this microorganism need to be addressed. Lactic acid bacteria (LAB) are a group of food-grade microorganisms that have been classified as safe by biologically regulatory agencies. In this context, we have demonstrated herein, for the first time, the production of fully functional, IB-like protein nanoparticles in LAB. These nanoparticles have been fully characterized using a wide range of techniques, including field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), dynamic light scattering (DLS), Fourier transform infrared (FTIR) spectroscopy, zymography, cytometry, confocal microscopy, and wettability and cell coverage measurements. Our results allow us to conclude that these materials share the main physico-chemical characteristics with IBs from E. coli and moreover are devoid of any harmful endotoxin contaminant. These findings reveal a new platform for the production of protein-based safe products with high pharmaceutical interest. |
dc.format.extent | 10 p. |
dc.language.iso | eng |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/es/ |
dc.subject | Àrees temàtiques de la UPC::Matemàtiques i estadística::Estadística aplicada::Estadística biosanitària |
dc.subject | Àrees temàtiques de la UPC::Matemàtiques i estadística::Probabilitat |
dc.subject | Àrees temàtiques de la UPC::Matemàtiques i estadística::Matemàtica discreta::Combinatòria |
dc.subject.lcsh | Combinatorial probabilities |
dc.subject.lcsh | Numerical analysis--Simulation methods |
dc.subject.lcsh | Genetics |
dc.subject.other | Endotoxin-free |
dc.subject.other | Functional nanomaterials |
dc.subject.other | GRAS |
dc.subject.other | Lactic acid bacteria |
dc.subject.other | Nanoparticles |
dc.title | Functional protein-based nanomaterial produced in microorganisms recognized as safe: A new platform for biotechnology |
dc.type | Article |
dc.subject.lemac | Probabilitats |
dc.subject.lemac | Anàlisi numèrica |
dc.subject.lemac | Genètica |
dc.contributor.group | Universitat Politècnica de Catalunya. gAGE - Grup d'Astronomia i Geomàtica |
dc.identifier.doi | 10.1016/j.actbio.2016.07.038 |
dc.description.peerreviewed | Peer Reviewed |
dc.subject.ams | Classificació AMS::60 Probability theory and stochastic processes::60C05 Combinatorial probability |
dc.subject.ams | Classificació AMS::65 Numerical analysis::65C Probabilistic methods, simulation and stochastic differential equations |
dc.subject.ams | Classificació AMS::92 Biology and other natural sciences::92D Genetics and population dynamics |
dc.relation.publisherversion | http://www.sciencedirect.com/science/article/pii/S1742706116303725 |
dc.rights.access | Open Access |
local.identifier.drac | 18850460 |
dc.description.version | Postprint (published version) |
dc.relation.projectid | info:eu-repo/grantAgreement/EC/H2020/654360/EU/NANOSCIENCE FOUNDRIES AND FINE ANALYSIS/NFFA-Europe |
local.citation.author | Cano, O.; Sanchez, A.; Parés, S.; Giró, I.; Tatkiewicz, W.; Ferrer-Miralles, N.; Ratera, I.; Natalello, A.; Cubarsi, R.; Veciana, J.; Bach, A.; Villaverde, A.; Aris, A.; García, E. |
local.citation.publicationName | Acta biomaterialia |
local.citation.volume | 43 |
local.citation.startingPage | 230 |
local.citation.endingPage | 239 |
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