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dc.contributor.authorCano Garrido, Olivia
dc.contributor.authorSanchez Chardi, Alejandro
dc.contributor.authorParés, Silvia
dc.contributor.authorGiró, Irene
dc.contributor.authorTatkiewicz, Witold I.
dc.contributor.authorFerrer Miralles, Neus
dc.contributor.authorRatera, Imma
dc.contributor.authorNatalello, Antonino
dc.contributor.authorCubarsí Morera, Rafael
dc.contributor.authorVeciana Miró, Jaume
dc.contributor.authorBach, Alex
dc.contributor.authorVillaverde Corrales, Antonio Pedro
dc.contributor.authorArís, Anna
dc.contributor.authorGarcía Fruitós, Elena
dc.contributor.otherUniversitat Politècnica de Catalunya. Departament de Matemàtiques
dc.date.accessioned2017-03-09T09:45:06Z
dc.date.available2018-10-02T00:30:30Z
dc.date.issued2016-10-01
dc.identifier.citationCano, O., Sanchez, A., Parés, S., Giró, I., Tatkiewicz, W., Ferrer-Miralles, N., Ratera, I., Natalello, A., Cubarsi, R., Veciana, J., Bach, A., Villaverde, A., Aris, A., García, E. Functional protein-based nanomaterial produced in microorganisms recognized as safe: A new platform for biotechnology. "Acta biomaterialia", 1 Octubre 2016, vol. 43, p. 230-239.
dc.identifier.issn1742-7061
dc.identifier.urihttp://hdl.handle.net/2117/102175
dc.description.abstractInclusion bodies (IBs) are protein-based nanoparticles formed in Escherichia coli through stereospecific aggregation processes during the overexpression of recombinant proteins. In the last years, it has been shown that IBs can be used as nanostructured biomaterials to stimulate mammalian cell attachment, proliferation, and differentiation. In addition, these nanoparticles have also been explored as natural delivery systems for protein replacement therapies. Although the production of these protein-based nanomaterials in E. coli is economically viable, important safety concerns related to the presence of endotoxins in the products derived from this microorganism need to be addressed. Lactic acid bacteria (LAB) are a group of food-grade microorganisms that have been classified as safe by biologically regulatory agencies. In this context, we have demonstrated herein, for the first time, the production of fully functional, IB-like protein nanoparticles in LAB. These nanoparticles have been fully characterized using a wide range of techniques, including field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), dynamic light scattering (DLS), Fourier transform infrared (FTIR) spectroscopy, zymography, cytometry, confocal microscopy, and wettability and cell coverage measurements. Our results allow us to conclude that these materials share the main physico-chemical characteristics with IBs from E. coli and moreover are devoid of any harmful endotoxin contaminant. These findings reveal a new platform for the production of protein-based safe products with high pharmaceutical interest.
dc.format.extent10 p.
dc.language.isoeng
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.subjectÀrees temàtiques de la UPC::Matemàtiques i estadística::Estadística aplicada::Estadística biosanitària
dc.subjectÀrees temàtiques de la UPC::Matemàtiques i estadística::Probabilitat
dc.subjectÀrees temàtiques de la UPC::Matemàtiques i estadística::Matemàtica discreta::Combinatòria
dc.subject.lcshCombinatorial probabilities
dc.subject.lcshNumerical analysis--Simulation methods
dc.subject.lcshGenetics
dc.subject.otherEndotoxin-free
dc.subject.otherFunctional nanomaterials
dc.subject.otherGRAS
dc.subject.otherLactic acid bacteria
dc.subject.otherNanoparticles
dc.titleFunctional protein-based nanomaterial produced in microorganisms recognized as safe: A new platform for biotechnology
dc.typeArticle
dc.subject.lemacProbabilitats
dc.subject.lemacAnàlisi numèrica
dc.subject.lemacGenètica
dc.contributor.groupUniversitat Politècnica de Catalunya. gAGE - Grup d'Astronomia i Geomàtica
dc.identifier.doi10.1016/j.actbio.2016.07.038
dc.description.peerreviewedPeer Reviewed
dc.subject.amsClassificació AMS::60 Probability theory and stochastic processes::60C05 Combinatorial probability
dc.subject.amsClassificació AMS::65 Numerical analysis::65C Probabilistic methods, simulation and stochastic differential equations
dc.subject.amsClassificació AMS::92 Biology and other natural sciences::92D Genetics and population dynamics
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S1742706116303725
dc.rights.accessOpen Access
local.identifier.drac18850460
dc.description.versionPostprint (published version)
dc.relation.projectidinfo:eu-repo/grantAgreement/EC/H2020/654360/EU/NANOSCIENCE FOUNDRIES AND FINE ANALYSIS/NFFA-Europe
local.citation.authorCano, O.; Sanchez, A.; Parés, S.; Giró, I.; Tatkiewicz, W.; Ferrer-Miralles, N.; Ratera, I.; Natalello, A.; Cubarsi, R.; Veciana, J.; Bach, A.; Villaverde, A.; Aris, A.; García, E.
local.citation.publicationNameActa biomaterialia
local.citation.volume43
local.citation.startingPage230
local.citation.endingPage239


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