CXCR4(+)-targeted protein nanoparticles produced in the food-grade bacterium Lactococcus lactis
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Lactococcus lactis is a Gram-positive (endotoxin-free) food-grade bacteria exploited as alternative to Escherichia coli for recombinant protein production. We have explored here for the first time the ability of this platform as producer of complex, self-assembling protein materials. Materials & methods: Biophysical properties, cell penetrability and in vivo biodistribution upon systemic administration of tumor-targeted protein nanoparticles produced in L. lactis have been compared with the equivalent material produced in E. coli. Results: Protein nanoparticles have been efficiently produced in L. lactis, showing the desired size, internalization properties and biodistribution. Conclusion: In vitro and in vivo data confirm the potential and robustness of the production platform, pointing out L. lactis as a fascinating cell factory for the biofabrication of protein materials intended for therapeutic applications.
CitationCano, O., Cespedes, M., Unzueta, U., Roldan, M., Cubarsi, R., Vazquez, E., García, E., Villaverde, A. CXCR4(+)-targeted protein nanoparticles produced in the food-grade bacterium Lactococcus lactis. "Nanomedicine", 1 Setembre 2016, vol. 18, núm. 18, p. 2387-2398.