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Cetacean ultrastructural cochlear imaging through scanning electron microscopy

Cita com:
hdl:2099/9236
Document typeArticle
Defense date2010-07-20
PublisherSARTI (Technological Development Centre of Remote Acquisition and Data processing Systems)
Rights accessOpen Access
This work is protected by the corresponding intellectual and industrial property rights.
Except where otherwise noted, its contents are licensed under a Creative Commons license
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Attribution-NonCommercial-NoDerivs 3.0 Spain
Abstract
The control of noise interaction between artificial and biological sources
is essential to assess the development of sustainable marine technologies. Therefore,
there is an emergent need to conduct morphological analysis of the acoustic
pathways of marine organisms and detect possible structural alterations as a
consequence of sound exposure. Cetaceans, because of their use of sounds in their
daily activities, represent today the best bioindicators of the acoustic balance of the
oceans. To access this information it is necessary to extract the ears of very fresh
stranded individuals. One of the challenging steps after extraction and fixation of
the samples is to decalcify the bone envelope to access the cochlea without damaging
the soft tissues. A fast commercial decalcifier (RDO®) was used in 93 ears from 11
different odontocete species stranded in the Mediterranean Sea, the North Atlantic
and the North Sea. Depending on the tympanic-periotic volume of the species, the
decalcification time ranged from several hours to a few days, instead of taking few
months with other decalcification agents, allowing a subsequently faster observation
of the cochlear structures. Here we present images from cetacean cochlear
ultrastructure through scanning electron microscopy. Following this protocol it is
possible to obtain a fast diagnostic of possible acoustic trauma and relate the results
to documented sound exposure. The output of this analysis will help calibrating
theoretical results derived from deep-sea observatories.
CitationMorell, Maria [et al.]. Cetacean ultrastructural cochlear imaging through scanning electron microscopy. "Instrumentation Viewpoint", 20 Juliol 2010, núm. 8, p. 103-104.
ISSN1886-4864
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