Yttria-stabilised tetragonal zirconia polycrystals (3Y-TZP) bioinert ceramics combine excellent strength and toughness, good aesthetics, high resistance to corrosion and absence of allergic reaction. However, improved osseointegration is needed as higher marginal bone loss was sometimes reported. In the present work, 3Y-TZP multi-patterned samples for rapid screening of cell-surface interactions were fabricated by femtosecond laser micromachining. Pits with well-defined edges and micrometric precision in pit diameter, depth and spacing were produced, as determined by white light interferometry. Pits showed a nanometric granular texture on the sidewalls and ripples at pit bottom, as attested by scanning electron microscopy. Focused ion beam analyses indicated limited laser-induced damage. Micropatterns impacted human mesenchymal stem cell (hMSC) size and morphology. Cell area and aspect ratio were mainly influenced by pit diameter, while solidity and circularity were affected by both pit diameter and depth. The pattern 30. µm diameter/10. µm depth induced the strongest osteoblastic hMSC commitment.