This paper presents a study of mechanical properties of Fused Deposition Modeling (FDM) rapid prototyping processed material Timberfill. In this study, four important process parameters such as layer thickness, density, printing velocity, and orientation are considered. Experiments are conducted based on L27 Taguchi orthogonal array for the design of experiment (DOE) in order to reduce experimental runs. Finally, the influence of parameters on the responses such as Young modulus parameters, elastic limit, elastic elongation, maximum tension, breaking stress and elongation at break of test specimen are studied. The validity of the models is tested using analysis of variance (ANOVA). The layer height has a practically linear tendency, increasing the value of the tension maximum when the layer height is higher. A maximum value of 585 MPa/kg has been obtained and a minimum of 505 MPa/kg.