The Remote sensing and Interference detector with radiome-Try and vegetation Analysis (RITA Payload) [1] is a development of the UPC NanoSat Lab, aimed at Earth Observation (EO). It is one of the winners of the Second IEEE GRSS Student Grand Challenge, and will fly on-board AlainSat-1, a 3U CubeSat developed by the National Space Science and Technology Center (NSSTC) of the United Arab Emirates.RITA hosts three experiments. An L-band microwave radiometer (MWR) will gather data of soil moisture and sea ice thickness and concentration, aided with a Radio-frequency Interference (RFI) detection algorithm. A LoRa transceiver will perform on-demand execution of the EO experiments [2]. Finally, a Near-Infrared (NIR) Hyperspectral Camera will gather data for vegetation monitoring, agriculture applications, hydrology and coastal and inland waters mapping, among others [3].This work is focused on the calibration and validation of the Hyperspectral imager, at optical, electronic and spectral levels, as well as in the verification of its performance to measure Normalized Difference Vegetation Index (NDVI).