Intrinsically colored cotton is crucial for sustainability as it eliminates the need for chemical dyes, reducing water pollution and carbon footprint. It also preserves biodiversity by using fewer pesticides and supports eco-friendly, ethical product creation. This research aims to examine the factors that influence the color change that occurs in naturally colored organic cotton (NaCOC) fabrics when washed under normal household conditions, and it focuses on special detergents designed for people with skin hypersensitivity. The study observes the impact of various washing conditions on the color changes of the fabrics. Specifically, three specific detergents, two types of water (tap and distilled), and three different temperatures (20, 40, and 60 °C) are taken into consideration as variables. By using colorimetric measures and correlating the results with the significant variables of the experimental design, the study evaluates how washing practices affect both the color and the overall integrity of the fabric. The findings demonstrate that the water hardness is the most influential variable when it comes to the color changes in the fabrics. Additionally, higher washing temperatures exacerbate color changes, particularly in hard water conditions. These results provide valuable insights for maintaining the color integrity of NaCOC fabrics during washing