The interest in tissue engineering is growing in the scientific and medical communities, as it can solve actual problems regarding donor tissues, wound healing and drug delivery systems. Nanofibers are gaining relevance in this topic thanks to their excellent mechanical properties and similarities concerning the human skin. This project has explored how combining the nanofibers’ membranes created of PLA, made by electrospinning, with a dissolution of lanoline and cinnamon essential oil not only imitates the human skin, as it was demonstrated in a later project, but also obtains an antibacterial character. Analytical techniques such as a spectrophotometer, an electrokinetic analyzer, a scanning electron microscope, a Fourier transform infrared spectroscope, and an optical tensiometer were employed. Results confirmed successful integration and migration of the cinnamon oil, with antibacterial efficacy achieved against specific bacterial strains, as hypothesized. Notably, scaffolds composed of seven layers exhibited migration behavior closely aligned with theoretical expectations