Now showing items 1-6 of 6

    • Bottom-Up instructive quality control in the biofabrication of smart protein materials 

      Rueda, Fabian; Cespedes, Maria Virtudes; Cochinillo, Oscar; Sanchez Chardi, Alejandro; Seras Franzoso, Joaquin; Cubarsí Morera, Rafael; Gallardo, Alberto; Pesarrodona, Mireia; Ferrer Miralles, Neus; Daura, Xavier; Vazquez Gómez, Esther; García Fruitós, Elena; Mangues, Ramón; Unzueta, Ugutz; Villaverde, A. (2015-10)
      Article
      Open Access
    • CXCR4(+)-targeted protein nanoparticles produced in the food-grade bacterium Lactococcus lactis 

      Cano Garrido, Olivia; Cespedes, Maria Virtudes; Unzueta, Ugutz; Roldan, Monica; Cubarsí Morera, Rafael; Vazquez Gómez, Esther; García Fruitós, Elena; Villaverde Corrales, Antonio Pedro (2016-09-01)
      Article
      Open Access
      Lactococcus lactis is a Gram-positive (endotoxin-free) food-grade bacteria exploited as alternative to Escherichia coli for recombinant protein production. We have explored here for the first time the ability of this ...
    • Endosomal escape of protein nanoparticles engineered through humanized histidine-rich peptides 

      Lopez Laguna, Hector; Cubarsí Morera, Rafael; Unzueta, Ugutz; Mangues, Ramón; Vazquez, Esther; Villaverde, Antonio (2019-12-27)
      Article
      Open Access
      Poly-histidine peptides such as H6 (HHHHHH) are used in protein biotechnologies as purification tags, pro- tein-assembling agents and endosomal-escape entities. The pleiotropic properties of such peptides make them appealing ...
    • Internalization and kinetics of nuclear migration of protein-only, arginine-rich nanoparticles 

      Vázquez, Esther; Cubarsí Morera, Rafael; Unzueta, Ugutz; Roldán, Mónica; Domingo-Espín, Joan; Ferrer-Miralles, Neus; Villaverde, Antonio (2010-12)
      Article
      Restricted access - publisher's policy
      Understanding the intracellular trafficking of nanoparticles internalized by mammalian cells is a critical issue in nanomedicine, intimately linked to therapeutic applications but also to toxicity concerns. While the uptake ...
    • Self-assembling as regular nanoparticles dramatically minimizes photobleaching of tumour-targeted GFP 

      Unzueta, Ugutz; Roldán Molina, Mónica; Pesarrodona, Mireia; Benítez Iglesias, Raúl; Sanchez Chardi, Alejandro; Conchillo, Oscar; Mangues, Ramón; Villaverde, Antonio; Vazquez Gómez, Esther (Elsevier, 2020-02-03)
      Article
      Open Access
      Fluorescent proteins are useful imaging and theranostic agents, but their potential superiority over alternative dyes is weakened by substantial photobleaching under irradiation. Enhancing protein photostability has been ...
    • Switching cell penetrating and CXCR4-binding activities of nanoscale-organized arginine-rich peptides 

      Teixeira De Pinho Favaro, Marianna; Serna, Naroa; Sanchez Garcia, Laura; Cubarsí Morera, Rafael; Roldan, Monica; Sanchez Chardi, Alejandro; Unzueta, Ugutz; Mangues, Ramón; Ferrer Miralles, Neus; Rodrigues Azzoni, Adriano; Vazquez Gómez, Esther; Villaverde, Antonio (2018-08-01)
      Article
      Open Access
      Arginine-rich protein motifs have been described as potent cell-penetrating peptides (CPPs) but also as rather specific ligands of the cell surface chemokine receptor CXCR4, involved in the infection by the human ...