Now showing items 1-6 of 6

  • Computer-aided laccase engineering: toward biological oxidation of arylamines 

    Santiago, Gerard; de Salas, Felipe; Lucas, Fatima; Monza, Emanuele; Acebes, Sandra; Martinez, Ángel T.; Camarero, Susana (ACS Publications, 2016-07-08)
    Article
    Open Access
    Oxidation of arylamines, such as aniline, is of high industrial interest and laccases have been proposed as biocata-lysts to replace harsh chemical oxidants. However, the reaction is hampered by the redox potential of the ...
  • Controlled manipulation of enzyme specificity through immobilization-induced flexibility constraints 

    Coscolín, Cristina; Beloqui, Ana; Martínez-Martínez, Mónica; Bargiela, Rafael; Santiago, Gerard; Blanco, Rosa M.; Delaittre, Guillaume; Márquez-Álvarez, Carlos; Ferrer, Manuel (Elsevier, 2018-09-05)
    Article
    Open Access
    It is thought that during immobilization enzymes, as dynamic biomolecules, may become distorted and this may alter their catalytic properties. However, the effects of different immobilization strategies on enzyme rigidity ...
  • Esterases computational study 

    Cañadas, Ruben; Santiago, Gerard; Guallar, Victor (Barcelona Supercomputing Center, 2017-05-04)
    Conference report
    Open Access
    In our project, we are focusing on two different esterases called LAE6 and LAE5. LAE6 is very promiscuous since it presents activity towards a wide range of different substrates, whereas LAE5 presents activity towards few ...
  • Protein modelling for enzyme engineering 

    Santiago, Gerard; Guallar, Victor (Barcelona Supercomputing Center, 2017-05-04)
    Conference report
    Open Access
  • Rational Engineering of Multiple Active Sites in an Ester Hydrolase 

    Santiago, Gerard; Martínez-Martínez, Mónica; Alonso, Sandra; Bargiela, Rafael; Coscolín, Cristina; Golyshing, Peter N.; Guallar, Victor; Ferrer, Manuel (American Chemical Society, 2018-03-30)
    Article
    Open Access
    Effects of altering the properties of an active site in an enzymatic homogeneous catalyst have been extensively reported. However, the possibility of increasing the number of such sites, as commonly done in heterogeneous ...
  • Re-designing the substrate binding pocket of laccase for enhanced oxidation of sinapic acid 

    Pardo, Isabel; Santiago, Gerard; Gentili, Patrizia; Lucas, Fátima; Monza, Emanuele; Medrano, Francisco Javier; Galli, Carlo; Martínez, Angel T.; Guallar, Víctor; Camarero, Susana (Royal Society of Chemistry, 2015-12-29)
    Article
    Open Access
    Iterative saturation mutagenesis was performed over six residues delimiting the substrate binding pocket of a high redox potential chimeric laccase with the aim of enhancing its activity over sinapic acid, a ligninrelated ...