Browsing by Subject "Microscòpia de fluorescència"
Now showing items 1-15 of 15
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Characterization of molecular orientation in super-resolution fluorescence microscopy
(Universitat Politècnica de Catalunya, 2012-09-06)
Master thesis
Open AccessIn this thesis we aim to characterize the effect of molecular dipole orientation on the resolution of super-resolution fluorescence microscopy techniques that rely on single molecule detection and localization. -
Development of microfluidic devices based on in 3D printing systems
(Universitat Politècnica de Catalunya, 2015-02)
Master thesis (pre-Bologna period)
Open AccessLos objetivos de este proyecto fueron la creación de un dispositivo microfluidico base en la impresión tres dimensiones, la obtención de un gradiente en este dispositivo, su control y observación. Para realizar el ... -
Fast image restoration in light-sheet fluorescence microscopy with extended depth of field using GPUs
(Universitat Politècnica de Catalunya, 2015-09-10)
Master thesis
Open AccessLight sheet fluorescence microscopy (LSFM) is used in many biological research experiments that require fast three-dimensional (3D) imaging up to a few volumes per second. Wavefront coding (WFC) microscopy provides LSFM ... -
High-throughput live imaging using Light Sheet Microscopy
(2020)
Conference report
Open AccessWe have developed a compact, multi-modal lightsheet microscope platform where different kind of experiments may be addressed with a single equipment. We will evaluate the performance of the Flexi-SPIM platform and its ... -
Imaging of human differentiated 3D neural aggregates using light sheet fluorescence microscopy
(2014-08-06)
Article
Open AccessThe development of three dimensional (3D) cell cultures represents a big step for the better understanding of cell behavior and disease in a more natural like environment, providing not only single but multiple cell type ... -
Light-sheet microscopy imaging of a whole cleared rat brain with Thy1-GFP transgene
(Nature, 2016-09)
Article
Open AccessWhole-brain imaging with light-sheet fluorescence microscopy and optically cleared tissue is a new, rapidly developing research field. Whereas successful attempts to clear and image mouse brain have been reported, a ... -
Multiphoton imaging microscopy at deeper layers with adaptive optics control of spherical aberration
(2013-07-17)
Article
Open AccessDespite the inherent confocality and optical sectioning capabilities of multiphoton microscopy, threedimensional (3-D) imaging of thick samples is limited by the specimen-induced aberrations. The combination of immersion ... -
Multiscale image analysis of calcium dynamics in cardiac myocytes
(Universitat Politècnica de Catalunya, 2017-09-22)
Doctoral thesis
Open AccessCardiac myocytes constitute a unique physiological system. They are the muscle cells that build up heart tissue and provide the force to pump blood by synchronously contracting at every beat. This contraction is regulated ... -
Multispectral imaging of healthy and diseased red blood cells using confocal microscopy
(2021-05-13)
Conference lecture
Open Access -
Nonlinear techniques in polarization based high resolution microscopy for selected biomedical samples
(Universitat Politècnica de Catalunya, 2015-09-10)
Master thesis
Open AccessHigh spatial resolution techniques, able to obtain fast images of structural changes in real time under label-free conditions, are required to understanding in vivo processes. Polarization sensitive second harmonic generation ... -
Polarized super-resolution fluorescence microscopy
(Universitat Politècnica de Catalunya, 2014-07-12)
Doctoral thesis
Open Access
Covenantee: Aix-Marseille UniversitéWhile super-resolution microscopy has brought a significant improvement in nano-scale imaging of molecular assemblies in biological media, its extension to imaging molecular orientation using fluorescence anisotropy has ... -
SPIM-fluid: open source light-sheet based platform for high-throughput imaging
(2015-11-01)
Article
Open AccessLight sheet fluorescence microscopy has recently emerged as the technique of choice for obtaining high quality 3D images of whole organisms/embryos with low photodamage and fast acquisition rates. Here we present an ... -
Use of photonic lanterns in light sheet microscopy applications
(Universitat Politècnica de Catalunya, 2020-06)
Bachelor thesis
36 months embargoLight-sheet fluorescence microscopy (LSFM) has proven to be a very useful technique at imaging living samples due to its low radiation doses and image acquisition speed. The light-sheet is usually produced by laser ... -
Viabilidad del estudio de la respuesta biológica en muestras opacas, utilizando microscopia correlativa
(Universitat Politècnica de Catalunya, 2014)
Master thesis
Open AccessLa implantología para la sustitución ósea es un sector industrial en auge como resultado del envejecimiento de la población en el primer mundo. La mayoría de los implantes para la sustitución de tejido duro se fabrican con ... -
Visual feature extraction to discriminate nucleolus phenotypes in fluorescence microscopy
(Universitat Politècnica de Catalunya, 2014-07-22)
Master thesis (pre-Bologna period)
Open Access
Covenantee: Université Catholique de Louvain[ANGLÈS] The objective of this project was the feature extraction using image processing tools over nucleolus isolated images of eukaryotic cells to subsequently classify between normal or healthy cells and abnormal or ...
