Single-molecule microscopy techniques have emerged as useful tools to image individual molecules and analyze their dynamics inside cells, but their application has mostly been restricted to cell cultures. Here, a light-sheet ...

In this thesis we aim to characterize the effect of molecular dipole orientation on the resolution of super-resolution fluorescence microscopy techniques that rely on single molecule detection and localization.

Light sheet fluorescence microscopy (LSFM) is used in many biological research experiments that require fast three-dimensional (3D) imaging up to a few volumes per second. Wavefront coding (WFC) microscopy provides LSFM ...

The development of three dimensional (3D) cell cultures represents a big step for the better understanding of cell behavior and disease in a more natural like environment, providing not only single but multiple cell type ...

Whole-brain imaging with light-sheet fluorescence microscopy and optically cleared tissue is a new, rapidly developing research field. Whereas successful attempts to clear and image mouse brain have been reported, a ...

Cardiac myocytes constitute a unique physiological system. They are the muscle cells that build up heart tissue and provide the force to pump blood by synchronously contracting at every beat. This contraction is regulated ...

High spatial resolution techniques, able to obtain fast images of structural changes in real time under label-free conditions, are required to understanding in vivo processes. Polarization sensitive second harmonic generation ...

Light sheet fluorescence microscopy has recently emerged as the technique of choice for obtaining high quality 3D images of whole organisms/embryos with low photodamage and fast acquisition rates. Here we present an ...

La implantología para la sustitución ósea es un sector industrial en auge como resultado del envejecimiento de la población en el primer mundo. La mayoría de los implantes para la sustitución de tejido duro se fabrican con ...