Now showing items 1-9 of 9

  • Aromatic stacking interactions govern catalysis in aryl-alcohol oxidase 

    Ferreira, Patricia; Hernández-Ortega, Aitor; Lucas, Fatima; Carro, Juan; Herguedas, Beatriz; Borrelli, Kenneth W.; Guallar, Víctor; Martínez, Angel T.; Medina, Milagros (FEBSPress, 2015-02-23)
    Article
    Open Access
    Aryl-alcohol oxidase (AAO, EC 1.1.3.7) generates H2O2 for lignin degradation at the expense of benzylic and other π system-containing primary alcohols, which are oxidized to the corresponding aldehydes. Ligand diffusion ...
  • Basidiomycete DyPs: Genomic diversity, structural–functional aspects, reaction mechanism and environmental significance 

    Linde, Dolores; Ruiz-Dueñas, Francisco J.; Fernandez-Fueyo, Elena; Guallar, Víctor; Hammel, Kenneth E.; Pogni, Rebecca; Martínez, Angel T. (Elsevier, 2015-05-15)
    Article
    Open Access
    The first enzyme with dye-decolorizing peroxidase (DyP) activity was described in 1999 from an arthroconidial culture of the fungus Bjerkandera adusta. However, the first DyP sequence had been deposited three years before, ...
  • Catalytic surface radical in dye-decolorizing peroxidase: A computational, spectroscopic and directed mutagenesis study 

    Linde, Dolores; Pogni, Rebecca; Cañellas, Marina; Lucas, Fátima; Guallar, Víctor; Ruiz-Dueñas, Francisco J.; Baratto, Maria Camilla; Sinocro, Adalgisa; Coscolin, Cristina; Romero, Antonio; Medrano, Francisco Javier; Martínez, Angel T. (Portland Press, 2015-03-01)
    Article
    Open Access
    Dye-decolorizing peroxidase (DyP) of Auricularia auriculajudae has been expressed in Escherichia coli as a representative of a new DyP family, and subjected to mutagenic, spectroscopic, crystallographic and computational ...
  • How experiments and molecular simulations can help understand selective C25-hydroxylation of vitamin D by fungal peroxygenases 

    Lucas, Fatima; Babot, Esteban D.; Cañellas, Marina; del Río, José C.; Kalum, Lisbeth; Ullrich, René; Hofrichter, Martin; Martínez, Angel T.; Gutiérrez, Ana (2016-02-01)
    Conference lecture
    Open Access
  • Improving the Oxidative Stability of a High Redox Potential Fungal Peroxidase by Rational Design 

    Sáez-Jiménez, Veronica; Acebes, Sandra; Guallar, Víctor; Martínez, Angel T.; Ruiz-Dueñas, Francisco Javier (PLoS, 2015-04-29)
    Article
    Open Access
    Ligninolytic peroxidases are enzymes of biotechnological interest due to their ability to oxidize high redox potential aromatic compounds, including the recalcitrant lignin polymer. However, different obstacles prevent ...
  • Insights on Laccase Engineering from Molecular Simulations: Towards a Binding Focused Strategy 

    Monza, Emanuele; Lucas, Fatima; Camarero, Susana; Alejaldre, Lorea C.; Martínez, Angel T.; Guallar, Víctor (ACS Publications, 2015-03-30)
    Article
    Open Access
    Understanding the molecular determinants of enzyme performance is of primary importance for the rational design of ad hoc mutants. A novel approach, which combines efficient conformational sampling and quick reactivity ...
  • Mapping the Long-Range Electron Transfer Route in Ligninolytic Peroxidases 

    Acebes, Sandra; Ruiz-Dueñas, Francisco J.; Toubes, Mario; Sáez-Jiménez, Veronica; Pérez-Boada, Marta; Lucas, M. Fatima; Martínez, Angel T.; Guallar, Victor (ACS Publications, 2017-04-04)
    Article
    Open Access
    Combining a computational analysis with site-directed mutagenesis, we have studied the long-range electron transfer pathway in versatile and lignin peroxidases, two enzymes of biotechnological interest that play a key role ...
  • Re-designing the substrate binding pocket of laccase for enhanced oxidation of sinapic acid 

    Pardo, Isabel; Santiago, Gerard; Gentili, Patrizia; Lucas, Fátima; Monza, Emanuele; Medrano, Francisco Javier; Galli, Carlo; Martínez, Angel T.; Guallar, Víctor; Camarero, Susana (Royal Society of Chemistry, 2015-12-29)
    Article
    Open Access
    Iterative saturation mutagenesis was performed over six residues delimiting the substrate binding pocket of a high redox potential chimeric laccase with the aim of enhancing its activity over sinapic acid, a ligninrelated ...
  • Switching the substrate preference of fungal aryl-alcohol oxidase: towards stereoselective oxidation of secondary benzyl alcohols 

    Serrano, Ana; Sancho, Ferran; Viña-González, Javier; Carro, Juan; Alcalde, Miguel; Guallar, Victor; Martínez, Angel T. (Royal Society of Chemistry, 2019-01-22)
    Article
    Open Access
    Oxidation of primary alcohols by aryl-alcohol oxidase (AAO), a flavoenzyme that provides H2O2 to fungal peroxidases for lignin degradation in nature, is achieved by concerted hydroxyl proton transfer and stereoselective ...