Now showing items 1-10 of 10

    • Aromatic stacking interactions govern catalysis in aryl-alcohol oxidase 

      Ferreira, Patricia; Hernández-Ortega, Aitor; Lucas, Fatima; Carro, Juan; Herguedas, Beatriz; Borrelli, Kenneth W.; Guallar, Víctor; Martínez, Angel T.; Medina, Milagros (FEBSPress, 2015-02-23)
      Article
      Open Access
      Aryl-alcohol oxidase (AAO, EC 1.1.3.7) generates H2O2 for lignin degradation at the expense of benzylic and other π system-containing primary alcohols, which are oxidized to the corresponding aldehydes. Ligand diffusion ...
    • Basidiomycete DyPs: Genomic diversity, structural–functional aspects, reaction mechanism and environmental significance 

      Linde, Dolores; Ruiz-Dueñas, Francisco J.; Fernandez-Fueyo, Elena; Guallar, Víctor; Hammel, Kenneth E.; Pogni, Rebecca; Martínez, Angel T. (Elsevier, 2015-05-15)
      Article
      Open Access
      The first enzyme with dye-decolorizing peroxidase (DyP) activity was described in 1999 from an arthroconidial culture of the fungus Bjerkandera adusta. However, the first DyP sequence had been deposited three years before, ...
    • Catalytic surface radical in dye-decolorizing peroxidase: A computational, spectroscopic and directed mutagenesis study 

      Linde, Dolores; Pogni, Rebecca; Cañellas, Marina; Lucas, Fátima; Guallar, Víctor; Ruiz-Dueñas, Francisco J.; Baratto, Maria Camilla; Sinocro, Adalgisa; Coscolin, Cristina; Romero, Antonio; Medrano, Francisco Javier; Martínez, Angel T. (Portland Press, 2015-03-01)
      Article
      Open Access
      Dye-decolorizing peroxidase (DyP) of Auricularia auriculajudae has been expressed in Escherichia coli as a representative of a new DyP family, and subjected to mutagenic, spectroscopic, crystallographic and computational ...
    • How experiments and molecular simulations can help understand selective C25-hydroxylation of vitamin D by fungal peroxygenases 

      Lucas, Fatima; Babot, Esteban D.; Cañellas, Marina; del Río, José C.; Kalum, Lisbeth; Ullrich, René; Hofrichter, Martin; Martínez, Angel T.; Gutiérrez, Ana (2016-02-01)
      Conference lecture
      Open Access
    • Improving the Oxidative Stability of a High Redox Potential Fungal Peroxidase by Rational Design 

      Sáez-Jiménez, Veronica; Acebes, Sandra; Guallar, Víctor; Martínez, Angel T.; Ruiz-Dueñas, Francisco Javier (PLoS, 2015-04-29)
      Article
      Open Access
      Ligninolytic peroxidases are enzymes of biotechnological interest due to their ability to oxidize high redox potential aromatic compounds, including the recalcitrant lignin polymer. However, different obstacles prevent ...
    • Insights on Laccase Engineering from Molecular Simulations: Towards a Binding Focused Strategy 

      Monza, Emanuele; Lucas, Fatima; Camarero, Susana; Alejaldre, Lorea C.; Martínez, Angel T.; Guallar, Víctor (ACS Publications, 2015-03-30)
      Article
      Open Access
      Understanding the molecular determinants of enzyme performance is of primary importance for the rational design of ad hoc mutants. A novel approach, which combines efficient conformational sampling and quick reactivity ...
    • Mapping the Long-Range Electron Transfer Route in Ligninolytic Peroxidases 

      Acebes, Sandra; Ruiz-Dueñas, Francisco J.; Toubes, Mario; Sáez-Jiménez, Veronica; Pérez-Boada, Marta; Lucas, M. Fatima; Martínez, Angel T.; Guallar, Victor (ACS Publications, 2017-04-04)
      Article
      Open Access
      Combining a computational analysis with site-directed mutagenesis, we have studied the long-range electron transfer pathway in versatile and lignin peroxidases, two enzymes of biotechnological interest that play a key role ...
    • Modulating Fatty Acid Epoxidation vs Hydroxylation in a Fungal Peroxygenase 

      Carro, Juan; González-Benjumea, Alejandro; Fernández-Fueyo, Elena; Aranda, Carmen; Guallar, Victor; Gutiérrez, Ana; Martínez, Angel T. (American Chemical Society, 2019-06-14)
      Article
      Open Access
      Unspecific peroxygenases (UPOs) are fungal secreted counterparts of the cytochrome P450 monooxygenases present in most living cells. Both enzyme types share the ability to perform selective oxygenation reactions. Moreover, ...
    • Re-designing the substrate binding pocket of laccase for enhanced oxidation of sinapic acid 

      Pardo, Isabel; Santiago, Gerard; Gentili, Patrizia; Lucas, Fátima; Monza, Emanuele; Medrano, Francisco Javier; Galli, Carlo; Martínez, Angel T.; Guallar, Víctor; Camarero, Susana (Royal Society of Chemistry, 2015-12-29)
      Article
      Open Access
      Iterative saturation mutagenesis was performed over six residues delimiting the substrate binding pocket of a high redox potential chimeric laccase with the aim of enhancing its activity over sinapic acid, a ligninrelated ...
    • Switching the substrate preference of fungal aryl-alcohol oxidase: towards stereoselective oxidation of secondary benzyl alcohols 

      Serrano, Ana; Sancho, Ferran; Viña-González, Javier; Carro, Juan; Alcalde, Miguel; Guallar, Victor; Martínez, Angel T. (Royal Society of Chemistry, 2019-01-22)
      Article
      Open Access
      Oxidation of primary alcohols by aryl-alcohol oxidase (AAO), a flavoenzyme that provides H2O2 to fungal peroxidases for lignin degradation in nature, is achieved by concerted hydroxyl proton transfer and stereoselective ...