Browsing by Subject "Fluorescence microscopy"
Now showing items 1-20 of 20
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3D volume rendering of invertebrates using light-sheet fluorescence microscopy
(2015-08)
Article
Open AccessLight-Sheet Fluorescence Microscopy (LSFM) has recently emerged as the technique of choice for obtaining high quality 3D images of whole organisms with low photo-damage and fast acquisition rates. Unlike conventional ... -
A computational diffusion model to study antibody transport within reconstructed tumor microenvironments
(2020-12)
Article
Open AccessAntibodies revolutionized cancer treatment over the past decades. Despite their successfully application, there are still challenges to overcome to improve efficacy, such as the heterogeneous distribution of antibodies ... -
Advanced nanoscopy tools to monitor intracellular membrane trafficking
(Universitat Politècnica de Catalunya, 2021-09-09)
Master thesis
Open AccessIntracellular membrane trafficking function is to transport proteins and other macromolecules by membrane bound vesicles throughout the cell and it is key for maintaining homeostasis and responding to signaling processes. ... -
Analysis of intracellular protein dynamics in living zebrafish embryos using light-sheet fluorescence single-molecule microscopy
(2021-10-01)
Article
Open AccessSingle-molecule microscopy techniques have emerged as useful tools to image individual molecules and analyze their dynamics inside cells, but their application has mostly been restricted to cell cultures. Here, a light-sheet ... -
Assessing the efficiency of Laser-Induced Breakdown Spectroscopy (LIBS) based sorting of post-consumer aluminium scrap
(Elsevier, 2022)
Article
Open AccessThe aluminium Twitch fraction of a Belgian recycling facility could be further sorted by implementing Laser-Induced Breakdown Spectroscopy (LIBS). To achieve this goal, the presented research identifies commercially ... -
Characterization of molecular orientation in super-resolution fluorescence microscopy
(Universitat Politècnica de Catalunya, 2012-09-06)
Master thesis
Open AccessIn this thesis we aim to characterize the effect of molecular dipole orientation on the resolution of super-resolution fluorescence microscopy techniques that rely on single molecule detection and localization. -
Development of microfluidic devices based on in 3D printing systems
(Universitat Politècnica de Catalunya, 2015-02)
Master thesis (pre-Bologna period)
Open AccessLos objetivos de este proyecto fueron la creación de un dispositivo microfluidico base en la impresión tres dimensiones, la obtención de un gradiente en este dispositivo, su control y observación. Para realizar el ... -
Fast image restoration in light-sheet fluorescence microscopy with extended depth of field using GPUs
(Universitat Politècnica de Catalunya, 2015-09-10)
Master thesis
Open AccessLight sheet fluorescence microscopy (LSFM) is used in many biological research experiments that require fast three-dimensional (3D) imaging up to a few volumes per second. Wavefront coding (WFC) microscopy provides LSFM ... -
High-throughput live imaging using Light Sheet Microscopy
(2020)
Conference report
Open AccessWe have developed a compact, multi-modal lightsheet microscope platform where different kind of experiments may be addressed with a single equipment. We will evaluate the performance of the Flexi-SPIM platform and its ... -
Imaging of human differentiated 3D neural aggregates using light sheet fluorescence microscopy
(2014-08-06)
Article
Open AccessThe development of three dimensional (3D) cell cultures represents a big step for the better understanding of cell behavior and disease in a more natural like environment, providing not only single but multiple cell type ... -
Light-sheet fluorescence microscopy for the in vivo study of microtubule dynamics in the zebrafish embryo
(2021-10-01)
Article
Open AccessDuring its first hours of development, the zebrafish embryo presents a large microtubule array in the yolk region, essential for its development. Despite of its size and dynamic behavior, this network has been studied only ... -
Light-sheet microscopy imaging of a whole cleared rat brain with Thy1-GFP transgene
(Nature, 2016-09)
Article
Open AccessWhole-brain imaging with light-sheet fluorescence microscopy and optically cleared tissue is a new, rapidly developing research field. Whereas successful attempts to clear and image mouse brain have been reported, a ... -
Multiphoton imaging microscopy at deeper layers with adaptive optics control of spherical aberration
(2013-07-17)
Article
Open AccessDespite the inherent confocality and optical sectioning capabilities of multiphoton microscopy, threedimensional (3-D) imaging of thick samples is limited by the specimen-induced aberrations. The combination of immersion ... -
Multispectral imaging of healthy and diseased red blood cells using confocal microscopy
(2021-05-13)
Conference lecture
Open Access -
Nonlinear techniques in polarization based high resolution microscopy for selected biomedical samples
(Universitat Politècnica de Catalunya, 2015-09-10)
Master thesis
Open AccessHigh spatial resolution techniques, able to obtain fast images of structural changes in real time under label-free conditions, are required to understanding in vivo processes. Polarization sensitive second harmonic generation ... -
SPIM-fluid: open source light-sheet based platform for high-throughput imaging
(2015-11-01)
Article
Open AccessLight sheet fluorescence microscopy has recently emerged as the technique of choice for obtaining high quality 3D images of whole organisms/embryos with low photodamage and fast acquisition rates. Here we present an ... -
Temperature Imaging around Plasmonic Structures
(Universitat Politècnica de Catalunya, 2010-06-03)
Master thesis
Open AccessIn the course of this work a novel microscope was constructed that is able to obtain a temperature map on the micro and nanoscale. The principle is demonstrated by presenting thermal maps of heated gold micro and nano ... -
Use of photonic lanterns in light sheet microscopy applications
(Universitat Politècnica de Catalunya, 2020-06)
Bachelor thesis
Restricted access - confidentiality agreementLight-sheet fluorescence microscopy (LSFM) has proven to be a very useful technique at imaging living samples due to its low radiation doses and image acquisition speed. The light-sheet is usually produced by laser ... -
Viabilidad del estudio de la respuesta biológica en muestras opacas, utilizando microscopia correlativa
(Universitat Politècnica de Catalunya, 2014)
Master thesis
Open AccessLa implantología para la sustitución ósea es un sector industrial en auge como resultado del envejecimiento de la población en el primer mundo. La mayoría de los implantes para la sustitución de tejido duro se fabrican con ... -
Visual feature extraction to discriminate nucleolus phenotypes in fluorescence microscopy
(Universitat Politècnica de Catalunya, 2014-07-22)
Master thesis (pre-Bologna period)
Open Access
Covenantee: Université Catholique de Louvain[ANGLÈS] The objective of this project was the feature extraction using image processing tools over nucleolus isolated images of eukaryotic cells to subsequently classify between normal or healthy cells and abnormal or ...
