Light-Sheet Fluorescence Microscopy (LSFM) has recently emerged as the technique of choice for obtaining high quality 3D images of whole organisms with low photo-damage and fast acquisition rates. Unlike conventional ...

In this thesis we aim to characterize the effect of molecular dipole orientation on the resolution of super-resolution fluorescence microscopy techniques that rely on single molecule detection and localization.

Light sheet fluorescence microscopy (LSFM) is used in many biological research experiments that require fast three-dimensional (3D) imaging up to a few volumes per second. Wavefront coding (WFC) microscopy provides LSFM ...

The development of three dimensional (3D) cell cultures represents a big step for the better understanding of cell behavior and disease in a more natural like environment, providing not only single but multiple cell type ...

Despite the inherent confocality and optical sectioning capabilities of multiphoton microscopy, threedimensional (3-D) imaging of thick samples is limited by the specimen-induced aberrations. The combination of immersion ...

High spatial resolution techniques, able to obtain fast images of structural changes in real time under label-free conditions, are required to understanding in vivo processes. Polarization sensitive second harmonic generation ...

In the course of this work a novel microscope was constructed that is able to obtain a temperature map on the micro and nanoscale. The principle is demonstrated by presenting thermal maps of heated gold micro and nano ...

La implantología para la sustitución ósea es un sector industrial en auge como resultado del envejecimiento de la población en el primer mundo. La mayoría de los implantes para la sustitución de tejido duro se fabrican con ...