Now showing items 1-10 of 10

    • Adaptive optics multiphoton microscopy 

      Artal, P; Gualda Manzano, Emilio José; Bueno García, Juan Manuel (2010)
      Conference report
      Open Access
      We have developed an adaptive optics multiphoton microscope. The multiphoton imaging system combines an ultrafast high-power laser, a scanning unit, a motorized Z-scan device and a photon-counting detector. The adaptive ...
    • Adaptive optics multiphoton microscopy to study ex vivo ocular tissues 

      Bueno García, Juan Manuel; Gualda Manzano, Emilio José; Artal Soriano, Pablo (2010)
      Article
      Restricted access - publisher's policy
      We develop an adaptive optics (AO) multiphoton microscope by incorporating a deformable mirror and a Hartmann-Shack wavefront sensor. The AO module operating in closed-loop is used to correct for the aberrations of the ...
    • Analysis of aberrations effect in nonlinear processes for femtosecond laser pulses 

      Romero Altimis, C.; Gualda Manzano, Emilio José; Artal Soriano, Pablo; Bueno García, Juan Manuel; Borrego Varillas, Rocío; Alonso Fernández, Benjamín; Méndez Valverde, Cruz; Rodríguez Vázquez de Aldana, Javier; Roso Franco, Luis (Optical Society of American (OSA), 2009)
      Conference report
      Restricted access - publisher's policy
      A method for the analysis and control of femtosecond pulse wavefronts generated in non-linear crystals by three-wave mixing processes is presented. The possibility to improve the efficiency of these processes is discussed.
    • Analysis of corneal stroma organization with wavefront optimized nonlinear microscopy 

      Bueno García, Juan Manuel; Gualda Manzano, Emilio José; Artal Soriano, Pablo (2011-06)
      Article
      Open Access
      To investigate the organization of stromal collagen in healthy ex vivo corneas of different species from second harmonic generation (SHG) microscopy images
    • Analysis of the chicken retina with an adaptive optics multiphoton microscope 

      Gualda Manzano, Emilio José; Artal Soriano, Pablo; Bueno García, Juan Manuel; Giakoumaki, Anastasia; Schaeffel, Frank (2011-06-01)
      Article
      Restricted access - publisher's policy
      The structure and organization of the chicken retina has been investigated with an adaptive optics multiphoton imaging microscope in a backward configuration. Non-stained flat-mounted retinal tissues were imaged at ...
    • Multiphoton imaging microscopy at deeper layers with adaptive optics control of spherical aberration 

      Bueno García, Juan Manuel; Palacios Higueras, Raquel; Gualda Manzano, Emilio José; Artal Soriano, Pablo; Skorsetz, Martin (2013-07-17)
      Article
      Open Access
      Despite the inherent confocality and optical sectioning capabilities of multiphoton microscopy, threedimensional (3-D) imaging of thick samples is limited by the specimen-induced aberrations. The combination of immersion ...
    • Multiphoton microscopy of ex vivo corneas after collagen cross-linking 

      Marcos Celestino, Susana; Gualda Manzano, Emilio José; Artal Soriano, Pablo; Bueno García, Juan Manuel; Giakoumaki, Anastasia; Pérez Merino, Pablo (2011-07-18)
      Article
      Open Access
      To investigate changes in the morphology of the corneal stroma after collagen cross-linking (CXL) treatment in bovine and porcine eyes using a nonlinear microscope providing both two-photon excitation fluorescence (TPEF) ...
    • Nonlinear 3D microscopy of ex vivo corneas 

      Gualda Manzano, Emilio José; Artal Soriano, Pablo; Bueno García, Juan Manuel (International Society for Photo-Optical Instrumentation Engineers (SPIE), 2010)
      Conference report
      Open Access
      A multiphoton microscope has been developed to investigate the sources of nonlinear fluorescence (TPEF) and second harmonic generation (SHG) in non-stained samples of ex-vivo corneas. Stacks of images from different depths ...
    • Retinal cell imaging in myopic chickens using adaptive optics multiphoton microscopy 

      Bueno García, Juan Manuel; Palacios Higueras, Raquel; Giakoumaki, Anastasia; Schaeffel, Frank; Gualda Manzano, Emilio José; Artal Soriano, Pablo (2014-03-01)
      Article
      Open Access
      Abnormal eye growth induced by visual deprivation can modify the structure and density of the retinal cells. We have used an adaptive optics multiphoton microscope to image photoreceptors (PRs) and ganglion cells (GCs) ...
    • Wavefront optimized nonlinear microscopy of ex vivo human retinas 

      Gualda Manzano, Emilio José; Artal Soriano, Pablo; Bueno García, Juan Manuel (2010)
      Article
      Restricted access - publisher's policy
      A multiphoton microscope incorporating a HartmannShack HS wavefront sensor to control the ultrafast laser beam’s wavefront aberrations has been developed. This instrument allowed us to investigate the impact of the laser ...