Now showing items 1-13 of 13

    • Compact ultrafast semiconductor disk laser: targeting GFP based nonlinear applications in living organisms 

      Loza Álvarez, Pablo; Artigas García, David; Santos, Susana I.C.O.; Aviles Espinosa, Rodrigo; Filippidis, George; Hamilton, Craig; Malcolm, Graeme; Weingarten, Kurt J.; Südmeyer, Thomas; Barbarin, Yohan; Keller, Ursula (2011-03-01)
      Article
      Restricted access - publisher's policy
      We present a portable ultrafast Semiconductor Disk Laser (SDL) (or vertical extended cavity surface emitting laser—VECSELs), to be used for nonlinear microscopy. The SDL is modelocked using a quantum-dot semiconductor ...
    • Decoupled illumination detection in light sheet microscopy for fast volumetric imaging 

      OLARTE, Omar Eduardo; Andilla, Jordi; Artigas García, David; Loza Álvarez, Pablo (Optical Society of American (OSA), 2015-08-04)
      Article
      Restricted access - publisher's policy
      Current microscopy demands the visualization of large threedimensional samples with increased sensitivity, higher resolution, and faster speed. Several imaging techniques based on widefield, point-scanning, and light-sheet ...
    • Depth aberrations characterization in linear and nonlinear microscopy schemes using a Shack-Hartmann wavefront sensor 

      Aviles Espinosa, Rodrigo; Andilla, Jordi; Porcar-Guezenec, Rafael; Levecq, Xavier; Artigas García, David; Loza Álvarez, Pablo (2012)
      Conference report
      Restricted access - publisher's policy
      The performance of imaging devices such as linear and nonlinear microscopes (NLM) can be limited by the optical properties of the imaged sample. Such an important aspect has already been described using theoretical models ...
    • Effect of molecular organization on the image histograms of polarization SHG microscopy 

      Psilodimitrakopoulos, Sotiris; Amat Roldán, Iván; Loza Álvarez, Pablo; Artigas García, David (2012)
      Article
      Open Access
      Based on its polarization dependency, second harmonic generation (PSHG) microscopy has been proven capable to structurally characterize molecular architectures in different biological samples. By exploiting this polarization ...
    • Estimating the helical pitch angle of amylopectin in starch using polarization second harmonic generation microscopy 

      Psilodimitrakopoulos, Sotiris; Amat Roldán, Iván; Loza Álvarez, Pablo; Artigas García, David (2010-08)
      Article
      Restricted access - publisher's policy
      Starch granules are among the brightest natural second harmonic generation (SHG) converters. They basically consist of amylose and amylopectin molecules and the source of the SHG signal is still undetermined. In the ...
    • Fast monitoring of in-vivo conformational changes in myosin using single scan polarization- SHG microscopy 

      Psilodimitrakopoulos, Sotiris; Loza Álvarez, Pablo; Artigas García, David (2014-12-01)
      Article
      Restricted access - publisher's policy
      Fast imaging of molecular changes under high-resolution and label-free conditions are essential for understanding in-vivo processes, however, current techniques are not able to monitor such changes in real time. Polarization ...
    • Imaging amylopectin's order in starch using 3-dimensional polarization SHG 

      Psilodimitrakopoulos, Sotiris; Amat Roldán, Iván; Artigas García, David; Loza Álvarez, Pablo (International Society for Photo-Optical Instrumentation Engineers (SPIE), 2011)
      Conference report
      Restricted access - publisher's policy
      In minimally destructive SHG biomedical imaging (high resolution optical slicing) is greatly desirable to extract the maximum of information from the light matter interaction. Here we develop a 3-D biophysical model and ...
    • Measurement and correction of in vivo sample aberrations employing a nonlinear guide-star in two-photon excited fluorescence microscopy 

      Aviles Espinosa, Rodrigo; Andilla, Jordi; Porcar-Guezenec, Rafael; Olarte, Omar E.; Nieto, Marta; Levecq, Xavier; Artigas García, David; Loza Álvarez, Pablo (2011-11-01)
      Article
      Open Access
      We demonstrate that sample induced aberrations can be measured in a nonlinear microscope. This uses the fact that two-photon excited fluorescence naturally produces a localized point source inside the sample: the nonlinear ...
    • Observation of self-focusing of light mediated by cubic nonlinearities in potassium titanyl phosphate 

      Carrasco Rodríguez, Sílvia; Kim, Hongki; Stegeman, George; Loza Álvarez, Pablo; Torner Sabata, Lluís (2002-11)
      Article
      Restricted access - publisher's policy
      We report our observations of the transient self-narrowing of light beams mediated by dominant Kerr nonlinearities and two-photon absorption in a bulk potassium titanyl phosphate crystal near conditions for second-harmonic ...
    • STED imaging performance estimation by means of Fourier transform analysis 

      Merino, David; Mallabiabarrena, Arrate; Andilla, Jordi; Artigas García, David; Zimmermann, Timo; Loza Álvarez, Pablo (2017-05-01)
      Article
      Open Access
      Due to relatively high powers used in STED, biological samples may be affected by the illumination in the process of image acquisition. Similarly, the performance of the system may be limited by the sample itself. Optimization ...
    • STED imaging performance estimation by means of Fourier transform analysis 

      Merino, David; Mallabiabarrena, Arrate; Andilla, Jordi; Artigas García, David; Zimmermann, Timo; Loza Álvarez, Pablo (OSA, 2017-04-17)
      Article
      Open Access
      Due to relatively high powers used in STED, biological samples may be affected by the illumination in the process of image acquisition. Similarly, the performance of the system may be limited by the sample itself. ...
    • Sub-diffraction discrimination with polarization-resolved two-photon excited fluorescence microscopy 

      Artigas García, David; Merino, David; Polzer, Christoph; Loza Álvarez, Pablo (Optical Society of American (OSA), 2017-08-20)
      Article
      Open Access
      Imaging molecular structures separated by distances of a few nanometers still represents a complex challenge. Moreover, it is normally restricted to observations on thin (few micrometers) samples. In this work, we rotate ...
    • Third-harmonic generation for the study of Caenorhabditis elegans embryogenesis 

      Aviles Espinosa, Rodrigo; Santos, Susana I.C.O.; Brodschelm, Andreas; Kaenders, Wilhelm G.; Alonso Ortega, Cesar; Artigas García, David; Loza Álvarez, Pablo (2010-08)
      Article
      Open Access
      Live microscopy techniques i.e., differential interference contrast, confocal microscopy, etc. have enabled the understanding of the mechanisms involved in cells and tissue formation. In long-term studies, special ...