Analysis of intracellular protein dynamics in living zebrafish embryos using light-sheet fluorescence single-molecule microscopy Bernardello, Matteo; Gualda Manzano, Emilio José; Castro Olvera, Gustavo; Loza Álvarez, Pablo (2021-10-01)
Open AccessSingle-molecule microscopy techniques have emerged as useful tools to image individual molecules and analyze their dynamics inside cells, but their application has mostly been restricted to cell cultures. Here, a light-sheet ...
Gualda Manzano, Emilio José; Bernardello, Matteo; Marsal, Maria; Loza Álvarez, Pablo (2020)
Open AccessWe have developed a compact, multi-modal lightsheet microscope platform where different kind of experiments may be addressed with a single equipment. We will evaluate the performance of the Flexi-SPIM platform and its ...
Light-sheet fluorescence microscopy for the in vivo study of microtubule dynamics in the zebrafish embryo Bernardello, Matteo; Marsal, Maria; Gualda Manzano, Emilio José; Loza Álvarez, Pablo (2021-10-01)
Open AccessDuring its first hours of development, the zebrafish embryo presents a large microtubule array in the yolk region, essential for its development. Despite of its size and dynamic behavior, this network has been studied only ...