Browsing by Author "Bernardello, Matteo"
Now showing items 1-6 of 6
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Analysis of intracellular protein dynamics in living zebrafish embryos using light-sheet fluorescence single-molecule microscopy
Bernardello, Matteo; Gualda Manzano, Emilio José; Castro Olvera, Gustavo; Loza Álvarez, Pablo (2021-10-01)
Article
Open AccessSingle-molecule microscopy techniques have emerged as useful tools to image individual molecules and analyze their dynamics inside cells, but their application has mostly been restricted to cell cultures. Here, a light-sheet ... -
Development of novel multimodal light-sheet fluorescence microscopes for in-vivo imaging of vertebrate organisms
Bernardello, Matteo (Universitat Politècnica de Catalunya, 2022-06-14)
Doctoral thesis
Open AccessThe observation of biological processes in their native environments is of critical importance for life science. While substantial information can be derived from the examination of in-vitro biological samples, in-vivo ... -
High-throughput live imaging using Light Sheet Microscopy
Gualda Manzano, Emilio José; Bernardello, Matteo; Marsal, Maria; Loza Álvarez, Pablo (2020)
Conference report
Open AccessWe have developed a compact, multi-modal lightsheet microscope platform where different kind of experiments may be addressed with a single equipment. We will evaluate the performance of the Flexi-SPIM platform and its ... -
Light-sheet fluorescence microscopy for the in vivo study of microtubule dynamics in the zebrafish embryo
Bernardello, Matteo; Marsal, Maria; Gualda Manzano, Emilio José; Loza Álvarez, Pablo (2021-10-01)
Article
Open AccessDuring its first hours of development, the zebrafish embryo presents a large microtubule array in the yolk region, essential for its development. Despite of its size and dynamic behavior, this network has been studied only ... -
Modular multimodal platform for classical and high throughput light sheet microscopy
Bernardello, Matteo; Gualda Manzano, Emilio José; Loza Álvarez, Pablo (Nature, 2022-02-04)
Article
Open AccessLight-sheet fluorescence microscopy (LSFM) has become an important tool for biological and biomedical research. Although several illumination and detection strategies have been developed, the sample mounting still represents ... -
Multiple asters organize the yolk microtubule network during dclk2-GFP zebrafish epiboly
Marsal, Maria; Bernardello, Matteo; Gualda Manzano, Emilio José; Loza, Pablo (Nature, 2022-03-08)
Article
Open AccessIt is known that the organization of microtubule (MT) networks in cells is orchestrated by subcellular structures named MT organizing centers (MTOCs). In this work, we use Light Sheet Fluorescence and Confocal Microscopy ...