Now showing items 1-14 of 14

    • Ageing is associated with deterioration of calcium homeostasis in isolated human right atrial myocytes 

      Herrainz, Adela; Álvarez-García, Jesus; Llach, A; Molina, Cristina E.; Fernandes, Jacqueline; Ferrero-Gregori, Andreu; Rodríguez, Cristina; Vallmitjana Lees, Alexander; Benítez Iglesias, Raúl; Padró, Josep M.; Martínez-González, José; Cinca Cosculluela, Juan; Hove-Madsen, Leif (2015-02-24)
      Article
      Open Access
      Ageing-related cardiac disorders such as heart failure and atrial fibrillation often present with intracellular calcium homeostasis dysfunction. However, knowledge of the intrinsic effects of ageing on cellular calcium ...
    • Cardiac electrical defects in progeroid mice and Hutchinson-Gilford progeria syndrome patients with nuclear lamina alterations 

      Riveras Torres, Jose; Calvo, Conrado J.; Llach, Anna; Gúzman Martínez, Gabriela; Caballero, Ricardo; González Gómez, Cristina; Jiménez Borreguero, Luís J.; Guadix, Juan A.; Osorio, Fernando G.; López Otín, Carlos; Herraiz Martínez, Adela; Cabello, Nuria; Vallmitjana Lees, Alexander; Benítez Iglesias, Raúl; Gordon, Leslie B. (2016-11-15)
      Article
      Open Access
      Hutchinson–Gilford progeria syndrome (HGPS) is a rare genetic disease caused by defective prelamin A processing, leading to nuclear lamina alterations, severe cardiovascular pathology, and premature death. Prelamin A ...
    • Detection of calcium release from individual RyR2 clusters in cardiomyocites 

      Nolla Colomer, Carme; Vallmitjana Lees, Alexander; Hove-Madsen, Leif; Benítez Iglesias, Raúl (2020-02)
      Article
      Restricted access - publisher's policy
      Cardiac ryanodine receptors (RyR2s) are Ca2þ release channels clustering in the sarcoplasmic reticulum membrane. These clusters are believed to be the elementary units of Ca2þ release. The distribution of these Ca2þ release ...
    • Distribution and function of cardiac ryanodine receptor clusters in live ventricular myocytes 

      Hiess, Florian; Vallmitjana Lees, Alexander; Wang, Ruiwu; Cheng, Hongqiang; ter Keurs, Henk; Chen, Ju; Hove Madsen, Leif; Benítez Iglesias, Raúl; Chen, S. R. Wayne (2015-08-14)
      Article
      Open Access
      The cardiac Ca2+ release channel (ryanodine receptor, RyR2) plays an essential role in excitation-contraction coupling in cardiac muscle cells. Effective and stable excitation-contraction coupling critically depends not ...
    • Dynamic and irregular distribution of RyR2 clusters in the periphery of live ventricular myocytes 

      Hiess, Florian; Detampel, Pascal; Nolla Colomer, Carme; Vallmitjana Lees, Alexander; Ganguly, Anutosh; Amrein, Matthias; ter Keurs, Henk E.D.J; Benítez Iglesias, Raúl; Hove-Madsen, Leif; Chen, S. R. Wayne (2018-01-23)
      Article
      Open Access
      Cardiac ryanodine receptors (RyR2s) are Ca2+ release channels clustering in the sarcoplasmic reticulum membrane. These clusters are believed to be the elementary units of Ca2+ release. The distribution of these Ca2+ release ...
    • Identification of intracellular calcium dynamics in stimulated cardiomyocytes 

      Vallmitjana Lees, Alexander; Barriga, Montserrat; Nenadic, Zoran; Llach, Anna; Álvarez Lacalle, Enrique; Hove-Madsen, Leif; Benítez Iglesias, Raúl (IEEE Press. Institute of Electrical and Electronics Engineers, 2010)
      Conference report
      Open Access
      We have developed an automatic method for the analysis and identification of dynamical regimes in intracellular calcium patterns from confocal calcium images. The method allows the identification of different dynamical ...
    • Low density lipoproteins promote unstable calcium handling accompanied by reduced SERCA2 and connexin-40 expression in cardiomyocytes 

      Barriga, Montserrat; Cal, Roi; Cabello, Nuria; Llach, Anna; Vallmitjana Lees, Alexander; Benítez Iglesias, Raúl; Badimón, Lina; Cinca, J.; Llorente-Cortés, Vicenta; Hove-Madsen, Leif (2013-03-13)
      Article
      Open Access
      The damaging effects of high plasma levels of cholesterol in the cardiovascular system are widely known, but little attention has been paid to direct effects on cardiomyocyte function. We therefore aimed at testing the ...
    • Multilevel analysis of calcium dynamics in stimulated cultures of cardiomyocytes 

      Vallmitjana Lees, Alexander; Hove-Madsen, Leif; Barriga, Montserrat; Benítez Iglesias, Raúl (Institute of Electrical and Electronics Engineers (IEEE), 2013)
      Conference report
      Restricted access - publisher's policy
      We present an automatic method to characterize calcium activity in a culture of cardiac cells from a sequence of microscopy fluorescence images. The approach quantifies both the response of each individual cell in the ...
    • Multiscale image analysis of calcium dynamics in cardiac myocytes 

      Vallmitjana Lees, Alexander (Universitat Politècnica de Catalunya, 2017-09-22)
      Doctoral thesis
      Open Access
      Cardiac myocytes constitute a unique physiological system. They are the muscle cells that build up heart tissue and provide the force to pump blood by synchronously contracting at every beat. This contraction is regulated ...
    • Phospholamban knockout breaks arrhythmogenic Ca2+ waves and suppresses catecholaminergic polymorphic ventricular tachycardia in mice 

      Bai, Yunlong; Jones, Peter; Guo, Jiqing; Zhong, Xiaowei; Clark, Robert; Zhou, Qiang; Wang, Ruiwu; Vallmitjana Lees, Alexander; Benítez Iglesias, Raúl; Hove-Madsen, Leif; Semeniuk, Lisa; Guo, Ang; Song, Long-Sheng; Duff, Henry J.; Chen, S.R. Wayne (2013-07-15)
      Article
      Restricted access - publisher's policy
      Rationale: Phospholamban (PLN) is an inhibitor of cardiac sarco(endo)plasmic reticulum Ca2+ ATPase. PLN knockout (PLN-KO) enhances sarcoplasmic reticulum Ca2+ load and Ca2+ leak. Conversely, PLN-KO accelerates Ca2+ ...
    • Prevention of adenosine A2A receptor activation diminishes beat-to-beat alternation in human atrial myocytes 

      Llacher Alsina, Anna Pilar; Vallmitjana Lees, Alexander; Benítez Iglesias, Raúl; Montiel Dacosta, José Antonio; Cinca Cusculluela, Juan (2016-01-01)
      Article
      Open Access
      © 2015, Springer-Verlag Berlin Heidelberg. Atrial fibrillation (AF) has been associated with increased spontaneous calcium release from the sarcoplasmic reticulum and linked to increased adenosine A2A receptor (A2AR) ...
    • Reduced expression of cardiac ryanodine receptor protects against stress-induced ventricular tachyarrhythmia, but increases the susceptibility to cardiac alternans 

      Zhong, Xiaowei; Vallmitjana Lees, Alexander; Sun, Bo; Xiao, Zhichao; Benítez Iglesias, Raúl (2018-01-15)
      Article
      Open Access
    • The 4q25 variant rs13143308T links risk of atrial fibrillation to defective calcium homoeostasis 

      Herraiz Martínez, Adela; Llach Martínez, Anna; Tarifa Lora, Carmen; Gandía, Jorge; Jiménez Sábado, Verónica; Lozano Velasco, Estefanía; Serra Pascual, Selma A.; Vallmitjana Lees, Alexander; Vázquez Ruiz de Castroviejo, Eduardo; Benítez Iglesias, Raúl (2019-03-01)
      Article
      Open Access
      Aims: Single nucleotide polymorphisms on chromosome 4q25 have been associated with risk of atrial fibrillation (AF) but the exiguous knowledge of the mechanistic links between these risk variants and underlying ...
    • The cardiac ryanodine receptor, but not sarcoplasmic reticulum Ca2-ATPase, is a major determinant of Ca2 alternans in intact mouse hearts 

      Sun, Bo; Wei, Jinhong; Zhong, Xiaowei; Vallmitjana Lees, Alexander; Benítez Iglesias, Raúl (2018-07-09)
      Article
      Open Access
      Sarcoplasmic reticulum (SR) Ca2+ cycling is governed by the cardiac ryanodine receptor (RyR2) and SR Ca2+-ATPase (SERCA2a). Abnormal SR Ca2+ cycling is thought to be the primary cause of Ca2+ alternans that can elicit ...