Elastin-like polymer coating on CoCr surfaces for cardiovascular applications
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Inclou dades d'ús des de 2022
Cita com:
hdl:2099.1/18830
Tipus de documentProjecte/Treball Final de Carrera
Data2013-02
Condicions d'accésAccés restringit per decisió de l'autor
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Abstract
Cardiovascular diseases have emerged as a major concern since they are the first cause of
death in developed countries. The advent of percutaneous transluminal coronary angioplasty
consisted in an alternative to invasive surgery. Especially the introduction of cardiovascular
stents has significantly meliorated the potential of angioplasty, e.g. with the use of drug
eluting and bioadsorbable stents. Still, restenosis remains a major implant-related
complication and can imply re intervention. Therefore, research has focused on limiting and/r
avoided restenosis by investigating the field of surface treatments and drug delivery material.
The rapidly developing field of material technology and engineering has enable design of
molecular-specific surfaces for a new generation of vascular devices. This project has been
focused on how to promote endothelialization on a bare metal CoCr alloy stent still widely
used nowadays. The knowledge about biological response and interactions between extra
cellular matrix proteins and cellular agents has lead to explore the potential of genetically
engineered protein based polymers such as elastin-like polymers which mimic properties of
the human elastin.
This project has for general goal to promote endothelialization on CoCr-alloy by
functionalizing the surfaces with REDV elastin-like biopolymer which contains a specific
domain for endothelial cell adhesion. For that purpose different surface treatments have been
performed on CoCr in order to enhance the biopolymer adhesion. First surfaces have been
activated by means of O2 plasma, acid HNO3 and NaOH basic etching. Afterwards, a series
has been CPTES silanized previous to biopolymer adsorption. Finally, REDV elastin-like
biopolymer has been physically and/or chemically attached to the different treated surfaces.
All treatments have been thoroughly physic-chemically characterized before and after all
treatments steps. Finally special attention has been paid to the study of biopolymer coating
stability after some thermal and/or mechanical treatments in order to determine the best
surface treatment conditions of adhesion and the efficiency of previous surface treatments.
REDV elastin-like biopolymer was successfully adsorbed on CoCr ASTM F-90 alloy surfaces.
It was biofunctionalized CoCr ASTM F-90 alloy surfaces through CPTES silane. Silanization
increased biopolymer adsorption but the efficiency was low. NaOH activated samples
presented a higher CPTES silane attachment and biopolymer adhesion as observed by an
increase of adhered HUVEC cells compared to other treatments.
REDV elastin-like biopolymer is more sensitive to mechanical and thermal treatments
probably due to a detachment and/or denaturation of the biomolecule. The increase of
silanization efficiency on treated CoCr surfaces could enhance biopolymer stability as
observed for NaOH treated surfaces.
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Fitxers | Descripció | Mida | Format | Visualitza |
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PFC_Anne-Sophie_Zenses.pdf | Report | 2,546Mb | Accés restringit |