Decoupled illumination detection in light sheet microscopy for fast volumetric imaging
Visualitza/Obre
did-optica-15.pdf (1,121Mb) (Accés restringit)
Sol·licita una còpia a l'autor
Què és aquest botó?
Aquest botó permet demanar una còpia d'un document restringit a l'autor. Es mostra quan:
- Disposem del correu electrònic de l'autor
- El document té una mida inferior a 20 Mb
- Es tracta d'un document d'accés restringit per decisió de l'autor o d'un document d'accés restringit per política de l'editorial
Cita com:
hdl:2117/87133
Tipus de documentArticle
Data publicació2015-08-04
EditorOptical Society of American (OSA)
Condicions d'accésAccés restringit per política de l'editorial
Llevat que s'hi indiqui el contrari, els
continguts d'aquesta obra estan subjectes a la llicència de Creative Commons
:
Reconeixement-NoComercial-SenseObraDerivada 3.0 Espanya
Abstract
Current microscopy demands the visualization of large threedimensional samples with increased sensitivity, higher resolution, and faster speed. Several imaging techniques based on widefield, point-scanning, and light-sheet strategies have been
designed to tackle some of these demands. Although successful, all these require the illuminated volumes to be tightly coupled with the detection optics to accomplish efficient optical sectioning. Here, we break this paradigm and produce
optical sections from out-of-focus planes. This is done by extending the depth of field of the detection optics in a lightsheet microscope using wavefront-coding techniques. This passive technique allows accommodation of the light sheet
at any place within the extended axial range. We show that this enables quick scanning of the light sheet across a volumetric sample. As a consequence, imaging speeds faster than twice the volumetric video rate (>70 volumes/s) can be
achieved without needing to move the sample. These capabilities are demonstrated for volumetric imaging of fast dynamics in vivo as well as for fast, three-dimensional particle tracking.
CitacióOmar Eduardo OLARTE, Andilla, J., Artigas, D., Loza, P. Decoupled illumination detection in light sheet microscopy for fast volumetric imaging. "Optica", 04 Agost 2015, vol. 2, núm. 8, p. 702-705.
ISSN2334-2536
Versió de l'editorhttps://www.osapublishing.org/optica/home.cfm
Fitxers | Descripció | Mida | Format | Visualitza |
---|---|---|---|---|
did-optica-15.pdf | 1,121Mb | Accés restringit |