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Micro-Infrared Spectroscopy (mSR-FTIR) and X-ray diffraction (mSR-XRD) with synchrotron light, Gas
Chromatography/Mass Spectrometry (CG/MS), Optical Microscopy (OM) and Scanning Electron
Microscopy (SEM/EDS) were used to identify and obtain the distribution of complex mixtures of calcium
salts of low molecular weight organic acids (LMWOA) in micro-layered micro-samples. Filamentous fungi
produce LMWOA that can react with metal cations producing stable salts. These substances were found
in the dark spots covering the surfaces of Saint Michael's Chapel wall paintings of the Royal Monastery of
Pedralbes in Barcelona linking them to old fungi activity. The presence of glycerol likewise related to the
fungi activity is also identified in the layers.
CitationSalvadó, N., Butí, S., Pradell, T., Beltran, V., Cinque, G., Juanhuix, J., Font, L., Senserrich, R. Low molecular weight organic acid salts, markers of old fungi activity in wall paintings. "Analytical Methods", 21 Febrer 2016, vol. 8, núm. 7, p. 1637-1645.
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