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dc.contributor.authorMestres, Gemma
dc.contributor.authorEspañol Pons, Montserrat
dc.contributor.authorxia, wei
dc.contributor.authorPersson, Cecilia
dc.contributor.authorGinebra Molins, Maria Pau
dc.contributor.authorKarlsson Ott, Marjam
dc.contributor.otherUniversitat Politècnica de Catalunya. Departament de Ciència dels Materials i Enginyeria Metal·lúrgica
dc.date.accessioned2016-03-31T11:28:02Z
dc.date.available2016-03-31T11:28:02Z
dc.date.issued2015-04-02
dc.identifier.citationMestres, G., Español, M., xia, W., Persson, C., Ginebra, M.P., Karlsson, M. Inflammatory response to nano- and microstructured hydroxyapatite. "PLoS one", 02 Abril 2015, vol. 10, núm. 4.
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/2117/84946
dc.description.abstractThe proliferation and activation of leukocytes upon contact with a biomaterial play a crucial role in the degree of inflammatory response, which may then determine the clinical failure or success of an implanted biomaterial. The aim of this study was to evaluate whether nano-and microstructured biomimetic hydroxyapatite substrates can influence the growth and activation of macrophage-like cells. Hydroxyapatite substrates with different crystal morphologies consisting of an entangled network of plate-like and needle-like crystals were evaluated. Macrophage proliferation was evaluated on the material surface (direct contact) and also in extracts i.e. media modified by the material (indirect contact). Additionally, the effect of supplementing the extracts with calcium ions and/or proteins was investigated. Macrophage activation on the substrates was evaluated by quantifying the release of reactive oxygen species and by morphological observations. The results showed that differences in the substrate's microstructure play a major role in the activation of macrophages as there was a higher release of reactive oxygen species after culturing the macrophages on plate-like crystals substrates compared to the almost non-existent release on needle-like substrates. However, the difference in macrophage proliferation was ascribed to different ionic exchanges and protein adsorption/retention from the substrates rather than to the texture of materials.
dc.language.isoeng
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.subjectÀrees temàtiques de la UPC::Enginyeria dels materials
dc.subject.lcshBiomedical materials
dc.subject.lcshHydroxyapatite
dc.subject.lcshNanoparticles
dc.subject.otherCalcium-phosphate cement
dc.subject.otherin-vitro
dc.subject.othercell response
dc.subject.othertricalcium phosphate
dc.subject.otherparticle-size
dc.subject.otherbone-marrow
dc.subject.othermacrophages
dc.subject.otherbiomaterials
dc.subject.othertopography
dc.subject.otheractivation
dc.titleInflammatory response to nano- and microstructured hydroxyapatite
dc.typeArticle
dc.subject.lemacBiomaterials
dc.subject.lemacHidroxiapatita
dc.subject.lemacNanopartícules
dc.contributor.groupUniversitat Politècnica de Catalunya. BBT - Biomaterials, Biomecànica i Enginyeria de Teixits
dc.identifier.doi10.1371/journal.pone.0120381
dc.description.peerreviewedPeer Reviewed
dc.relation.publisherversionhttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0120381
dc.rights.accessOpen Access
local.identifier.drac15620720
dc.description.versionPostprint (published version)
dc.relation.projectidinfo:eu-repo/grantAgreement/EC/FP7/291795/EU/GROWTH/GROWTH
local.citation.authorMestres, G.; Español, M.; xia, W.; Persson, C.; Ginebra, M.P.; Karlsson, M.
local.citation.publicationNamePLoS one
local.citation.volume10
local.citation.number4
dc.identifier.pmid25837264


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