Bacteriophage adsorption on electrospun scaffolds of PLA loaded with cationic arginine compounds
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hdl:2117/77347
Tipus de documentProjecte Final de Màster Oficial
Data2015-06
Condicions d'accésAccés obert
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Abstract
The research about biodegradable polymers has grown so quickly during last
decades. One of the main reasons is their application in the field of biomedicine.
These polymers are nontoxic for the organism and could be metabolized by the
human body after a controlled degradation. Gene therapy success depends on
safety and effective of the gene carriers. For that reason the creation of new
synthetic vectors is necessary. The temporary scaffolds are one of the most
important applications of biodegradable polymers. Temporary scaffolds are used
to replace a tissue of the human body that has been broken or weakened by an
illness, injury or surgery.
In this project new synthetized biodegradable polymers based on arginine are
employed and characterized through FTIR, RMN, DSC, X-rays and GPC.
Electrospun PLA scaffolds containing either this new family of polymers or pure
arginine are prepared. To obtain the optimal quality of the scaffold it is necessary
to tune the electrospinning operational parameters (e.g. flow rate, voltage,
needle-collector distance, collector type). Furthermore, both the conditions of the
solution (e.g. polymer type and concentration, viscosity, conductivity) as well as
the ambient parameters play an important role to get the optimal electrospinning
conditions.
The scaffolds are characterized with different techniques, SEM for the
morphologic study and fiber diameter measurement, contact angle to evaluate
the hydrophobicity of the surface, infrared analysis to assess the addition of the
different arginine compounds, and thermal analysis to determine variations on
thermal stability and characteristic transition temperatures.
The use of bacteriophages is an interesting alternative for drug-resistant
infections that has shown many good results in recent studies. The
bacteriophages are a type of virus that only infects bacteria.
Once characterized, the new prepared electrospun mats are loaded by adsorption
with bacteriophages, which are specific for the Staphylococcus Aureus bacteria.
The antibacterial ability of the loaded scaffolds is tested. PHMB is a well-known
antibacterial compound that is used as positive control. Biocompatibility of new
samples is also tested through cell adhesion and proliferation studies. Finally, the
potential use in biomedicine for tissue engineering and infection control is
evaluated.
MatèriesTissue scaffolds, Bacteriophages, Electrospinning, Biopolímers -- Microestructura, Teixits -- Bastides, Bacteriòfags, Electrofilatura, Biopolymers -- Microstructure
TitulacióMÀSTER UNIVERSITARI ERASMUS MUNDUS EN CIÈNCIA I ENGINYERIA DE MATERIALS AVANÇATS (Pla 2014)
Fitxers | Descripció | Mida | Format | Visualitza |
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Alejandro Lorenzo - Master Thesis.pdf | Report | 4,627Mb | Visualitza/Obre |