An efficient and reproducible method to culture Bergmann and cortical radial glia using textured PMMA
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Radial glia cells comprise the principal population of neural stem cells (NSC) during development. Attempts to develop reproducible radial glia and NSC culture methods have met with variable results, yielding non-adherent cultures or requiring the addition of growth factors. Recent studies demonstrated that a 2-µm patterned poly-methyl methacrylate (ln2 PMMA) grooved scaffold, by mimicking the biophysical and microtopographic properties of the embryonic NSC niche, induces the de-differentiation of glial cells into functional radial glia cell
CitationÁlvarez, Z. [et al.]. An efficient and reproducible method to culture Bergmann and cortical radial glia using textured PMMA. "Journal of neuroscience methods", 30 Juliol 2014, vol. 232, p. 93-101.