Knowledge of bacterial activity inside biofilms is key to the optimization of applied biotechnologies. A novel dissolved oxygen microelectrode array (DO-MEA) sensor has been designed and fabricated on a needle using microelectromechanical systems technology, in order to provide a multipoint, simultaneous dissolved oxygen snapshot profile inside a biofilm. The sensor consisted of eleven gold disk electrodes with integrated counter (CE) and pseudo-reference (RE) electrodes. Three different sensor designs are fabricated, and their responses are fully characterized and evaluated under different environmental conditions. The developed sensor is validated against a commercial Clark-type microelectrode overcoming its drawbacks, by profiling a heterotrophic biofilm cultivated in a flat-plate bioreactor. The DO-MEA sensor will be improved with the protection of the electrodes and will be fabricated on a thinner substrate, for becoming a powerful tool for the research of many similar biological-based processes and applications.
CitationMoya, A. [et al.]. Biofilm oxygen profiling using an array of microelectrodes on a microfabricated needle. A: Conference Eurosensors 2014 in Procedia Engineering. "Proceedings of the XXVIII Conference Eurosensors 2014 in Procedia Engineering". Brescia: 2014, p. 1-4.
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