Behavior of primary human osteoblasts on trimmed and sandblasted Ti6Al4V surfaces functionalized with integrin avß3-selective cyclic RGD peptides
Tipus de documentArticle
Condicions d'accésAccés restringit per política de l'editorial
It is well known that functionalization of surfaces with cell adhesive peptides mimicking the integrin binding motif of extracellular matrix proteins is a feasible approach to improve osseointegration of implant materials. Also, modi- fication of the surface properties of the material (e.g., rough- ness) strongly influences cell behavior. However, these two approaches are rarely studied together. This study addressed the hypothesis that the combination of peptide functionaliza- tion and surface roughness will have an enhancing effect on the adhesion process of osteoblasts. To test this hypothesis, a series of a v b 3-selective cyclic RGD peptides were prepared and immobilized on trimmed ( S a ¼ 0.74 l m, smooth) and sandblasted ( S a ¼ 3.24 l m, rough) Ti6Al4V disks. Effects of these surface modifications were evaluated with respect to integrin a v b 3-mediated adhesive capacity, cell morphology, and spreading of primary human osteoblasts. After 3 h of incubation, osteoblasts adhered more strongly on sand- blasted than on trimmed noncoated Ti6Al4V surfaces. Their attachment efficiency was further enhanced in the presence of RGD peptides. However, peptide functionalization had a relatively stronger impact on osteoblast attachment on trimmed surfaces compared with sandblasted surfaces. Cell morphology after 3 h of culture was exclusively altered by surface topography. RGD coating was critical for osteoblast spreading on both trimmed and sandblasted materials after 1 h of incubation but it showed almost negligible effects after 3 h. The results of this study provide evidence that the alli- ance of RGD coating and surface topography on Ti6Al4V pos- itively influences osteoblast adhesion and spreading, especially at very early adhesion times
CitacióMas-Moruno, C. [et al.]. Behavior of primary human osteoblasts on trimmed and sandblasted Ti6Al4V surfaces functionalized with integrin avß3-selective cyclic RGD peptides. "Journal of biomedical materials research. Part A", 2013, vol. 101, núm. 1, p. 87-97.
Versió de l'editorhttp://onlinelibrary.wiley.com/doi/10.1002/jbm.a.34303/abstract