Injectable collagen/alpha-tricalcium phosphate cement: collagen-mineral phase interactions and cell response
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A bone inspired material was obtained by incorporating collagen in the liquid phase of an a -trical- cium phosphate cement, either in solubilized or in fibrilized form. This material was able to set in situ, giving rise to a calcium deficient hydroxyapatite (CDHA)/collagen com- posite. The morphology and distribution of collagen in the composite was shown to be strongly affected by the col- lagen pre-treatment. The interactions between collagen and the inorganic phase were assessed by FTIR. A red shift of the amide I band was indicative of calcium chelation by the collagen carbonyl groups. The rate of CDHA formation was not affected when diluted collagen solutions (1 mg/ml) were used, whereas injectability improved. The presence of solubilized collagen, even in low amount (1 %), increased cell adhesion and proliferation on the composites. Still in the absence of osteogenic medium, significant ALP activity was detected both in the inorganic and the collagen-con- taining cements. The maximum ALP activity was advanced in the collagen-containing cement as compared to the inorganic cement.
CitacióPerez, R.A.; Ginebra, M.P. Injectable collagen/alpha-tricalcium phosphate cement: collagen-mineral phase interactions and cell response. "Journal of materials science. Materials in medicine", Febrer 2013, vol. 24, núm. 2, p. 381-393.