High production of poly(3-hydroxybutyrate) from a wild Bacillus megaterium Bolivian strain
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Tipus de documentArticle
Data publicació2013-02-26
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Abstract
Taking into account that a novel strain of
Bacillus megaterium
was
isolated from Uyuni salt lake (Bolivia) in a previous work, the objectives of
this new study were to determine the maximal Poly-3-hydroxybutyrate
production potential of
B. megaterium
strain uyuni S29 in an industrial
conventional media, the possibility that the strain accumulates different types
of polyhydroxyalkanoates, the cellular morphology during the biosynthesis
process and the characterization of the produced biopolymers.
Methods and Results:
The micro-organism was first tested in a 3-L bioreactor
obtaining a high specific growth rate of 1
64 h
1
. A second fed-batch experiment
was carried out in shaking flasks, reaching up to 70% PHB of cell dry mass. The
biosynthesized polymers were extracted by two different extraction procedures
and characterized. The results showed that all of them were PHB with thermal
properties different to the conventional PHB. The micrographs taken by TEM
show the different cell morphology during the fermentation process.
Conclusions:
In this previous study, the strain not only grew properly in the
industrial conditions proposed without spore formation, but also produced
and accumulated a large content of PHB, never reached before for its genus.
Therefore, if the culture conditions can be optimized, the biopolymer
production could be increased.
Significance and Impact of the Study:
The impact of the study has related to
the area of the biomaterials and their production. The study provides new data
related to the high production of PHB from the wild novel strain
B. megaterium
uyuni S29, the highest polymer accumulation for the genus
Bacillus
without spores formation.
CitacióRodriguez, A. [et al.]. High production of poly(3-hydroxybutyrate) from a wild Bacillus megaterium Bolivian strain. "Journal of applied microbiology", 26 Febrer 2013, vol. 114, núm. 5, p. 1378-1387.
ISSN1364-5072
Versió de l'editorhttp://onlinelibrary.wiley.com/doi/10.1111/jam.12151/abstract
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