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Ion-selective electrodes to monitor osteoblast-like cellular influence on the extracellular concentration of calcium
dc.contributor.author | Gustavsson, Johan |
dc.contributor.author | Planell Estany, Josep Anton |
dc.contributor.author | Engel López, Elisabeth |
dc.contributor.other | Universitat Politècnica de Catalunya. Departament de Ciència dels Materials i Enginyeria Metal·lúrgica |
dc.contributor.other | Institut de Bioenginyeria de Catalunya |
dc.date.accessioned | 2013-11-20T11:49:07Z |
dc.date.created | 2013-04-27 |
dc.date.issued | 2013-04-27 |
dc.identifier.citation | Gustavsson, J.; Planell, J.; Engel, E. Ion-selective electrodes to monitor osteoblast-like cellular influence on the extracellular concentration of calcium. "Journal of tissue engineering and regenerative medicine", 27 Abril 2013, vol. 7, núm. 8, p. 609-620. |
dc.identifier.issn | 1932-6254 |
dc.identifier.uri | http://hdl.handle.net/2117/20667 |
dc.description.abstract | In bone tissue engineering, the composition of the ionic extracellular environment (IEE) can determine both cellular fate and a biomaterial ’ s development and performance. Therefore, precise control of the IEE and a perfect understanding of the dynamic changes that it can be subject to due to cellular activity ishighlydesired.Toachievethis,weinitiallymonito redhowtwostandardosteoblast-likecellmodelsthat expressedeitherhighorlowalkalinephosphataseactivity-SAOS-2andMG63cells,respectively-affected the extracellular concentrations of calcium and phosphate during long-term cultures. It was observed that cellular in fl uence onthe IEE varied greatly between the two models and could be linked to the capac- ity of cells to deposit calcium in the extracellular matri x. Miniaturized ion-selective electrodes that could allow for real-time monitoring ofcalcium in aminimally invasive waywere then constructed.The electro- des were characterized in standard in vitro cell culture environments, prior to being successfully applied for periods of 24h, to record the dynamics of cell-induced deposition of calcium in the extracellular matrix, while using osteogenic media of either high or low concentrations of phosphate. As a result, this study provides the background and technological means for the non-destructive evaluation of the IEE in vitro and allows for the optimization and development of better models of bone tissue construction |
dc.format.extent | 12 p. |
dc.language.iso | eng |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Spain |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/es/ |
dc.subject | Àrees temàtiques de la UPC::Enginyeria dels materials |
dc.subject.lcsh | Tissue engineering |
dc.subject.lcsh | Osteoblasts |
dc.subject.other | Extracellular ions |
dc.subject.other | Ion-selective electrode |
dc.subject.other | MG63 |
dc.subject.other | Mineralization |
dc.subject.other | Osteoblasts |
dc.subject.other | Saos-2 |
dc.subject.other | Sensor |
dc.subject.other | Tissue engineering |
dc.title | Ion-selective electrodes to monitor osteoblast-like cellular influence on the extracellular concentration of calcium |
dc.type | Article |
dc.subject.lemac | Ossos -- Regeneració |
dc.subject.lemac | Enginyeria de teixits |
dc.contributor.group | Universitat Politècnica de Catalunya. BBT - Biomaterials, Biomecànica i Enginyeria de Teixits |
dc.identifier.doi | 10.1002/term.550 |
dc.description.peerreviewed | Peer Reviewed |
dc.relation.publisherversion | http://onlinelibrary.wiley.com/doi/10.1002/term.550/abstract |
dc.rights.access | Restricted access - publisher's policy |
local.identifier.drac | 12772291 |
dc.description.version | Postprint (published version) |
dc.relation.projectid | info:eu-repo/grantAgreement/EC/FP7/214402/EU/Angiogenesis-inducing Bioactive and Bioresponsive Scaffolds in Tissue Enginering/ANGIOSCAFF |
dc.date.lift | 10000-01-01 |
local.citation.author | Gustavsson, J.; Planell, J.; Engel, E. |
local.citation.publicationName | Journal of tissue engineering and regenerative medicine |
local.citation.volume | 7 |
local.citation.number | 8 |
local.citation.startingPage | 609 |
local.citation.endingPage | 620 |
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