Ion-selective electrodes to monitor osteoblast-like cellular influence on the extracellular concentration of calcium
Tipus de documentArticle
Condicions d'accésAccés restringit per política de l'editorial
In bone tissue engineering, the composition of the ionic extracellular environment (IEE) can determine both cellular fate and a biomaterial ’ s development and performance. Therefore, precise control of the IEE and a perfect understanding of the dynamic changes that it can be subject to due to cellular activity ishighlydesired.Toachievethis,weinitiallymonito redhowtwostandardosteoblast-likecellmodelsthat expressedeitherhighorlowalkalinephosphataseactivity-SAOS-2andMG63cells,respectively-affected the extracellular concentrations of calcium and phosphate during long-term cultures. It was observed that cellular in fl uence onthe IEE varied greatly between the two models and could be linked to the capac- ity of cells to deposit calcium in the extracellular matri x. Miniaturized ion-selective electrodes that could allow for real-time monitoring ofcalcium in aminimally invasive waywere then constructed.The electro- des were characterized in standard in vitro cell culture environments, prior to being successfully applied for periods of 24h, to record the dynamics of cell-induced deposition of calcium in the extracellular matrix, while using osteogenic media of either high or low concentrations of phosphate. As a result, this study provides the background and technological means for the non-destructive evaluation of the IEE in vitro and allows for the optimization and development of better models of bone tissue construction
CitacióGustavsson, J.; Planell, J.; Engel, E. Ion-selective electrodes to monitor osteoblast-like cellular influence on the extracellular concentration of calcium. "Journal of tissue engineering and regenerative medicine", 27 Abril 2013, vol. 7, núm. 8, p. 609-620.
Versió de l'editorhttp://onlinelibrary.wiley.com/doi/10.1002/term.550/abstract