A functional DNAmicroarray was prepared through the laser-induced forward transfer (LIFT) technique. In a first experiment, droplets of a buffer solution were spotted onto a substrate at different laser pulse energies. This allowed one to determine that uniform spots with a diameter as small as 40μm could be obtained. In a second experiment, a microarray containing two different human cDNAs and a negative control was spotted through LIFT and submitted to a hybridization assay. The obtained results demonstrated the full functionality of the microarray, which allowed us to prove the viability of LIFT for the production of DNAmicroarrays.
CitationSerra, P. [et al.]. Preparation of functional DNA microarrays through laser-induced forward transfer. "Applied physics letters", 30 Agost 2004, vol. 85, núm. 9, p. 1639-1641.
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