Osteoblast-like cellular response to dynamic changes in the ionic extracellular environment produced by calcium-deficient hydroxyapatite
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Tipus de documentArticle
Data publicació2012-10
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Abstract
Solution-mediated reactions due to ionic substitutions
are increasingly explored as a strategy to improve
the biological performance of calcium phosphate-based
materials. Yet, cellular response to well-defined dynamic
changes of the ionic extracellular environment has so far
not been carefully studied in a biomaterials context. In this
work, we present kinetic data on how osteoblast-like
SAOS-2 cellular activity and calcium-deficient hydroxyapatite
(CDHA) influenced extracellular pH as well as
extracellular concentrations of calcium and phosphate in
standard in vitro conditions. Since cells were grown on
membranes permeable to ions and proteins, they could
share the same aqueous environment with CDHA, but still
be physically separated from the material. In such culture
conditions, it was observed that gradual material-induced
adsorption of calcium and phosphate from the medium had
only minor influence on cellular proliferation and alkaline
phosphatase activity, but that competition for calcium and
phosphate between cells and the biomaterial delayed and
reduced significantly the cellular capacity to deposit calcium
in the extracellular matrix. The presented work thus
gives insights into how and to what extent solution-mediated
reactions can influence cellular response, and this will be
necessary to take into account when interpreting CDHA
performance both in vitro and in vivo.
CitacióGustavsson, J. [et al.]. Osteoblast-like cellular response to dynamic changes in the ionic extracellular environment produced by calcium-deficient hydroxyapatite. "Journal of materials science. Materials in medicine", Octubre 2012, vol. 23, núm. 10, p. 2509-2520.
ISSN0957-4530
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10.1007_s10856-012-4705-4.pdf | 2,335Mb | Accés restringit |